氢溴酸槟榔碱通过中枢神经迷走胆碱能系统促进犬胃肠运动  

Arecoline Hydrobromide Promotes Gastrointestinal Motility in Dogs through Central Nervous Vagal Cholinergic System

作　　者：陈其城 蒋志 张骏鸿 叶飞[3] 曹立幸 CHEN Qi-cheng;JIANG Zhi;ZHANG Jun-hong(Research Team of TCM Applications of Perioperative,Guangdong Academy of Traditional Chinese Medicine,Guangzhou(510120),China)

机构地区：[1]广东省中医药科学院围手术期中医药应用研究团队,广州510120 [2]广东省中医药科学院公共实验平台,广州510120 [3]广东省中医院大学城医院麻醉科,广州510120

出　　处：《中国中西医结合外科杂志》2022年第5期683-689,共7页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：国家区域中医(专科)诊疗中心建设专项资金资助(国中医药医政函(2018)205号广东省中医院外科);广东省中医院临床研究专项(YN10101902、YN10101911);广州中医药大学“双一流”与高水平大学学科协同创新团队项目(2021xk48)。

摘　　要：目的:探讨氢溴酸槟榔碱增强胃肠运动作用的中枢神经机制。方法:在比格犬胃体、胃窦、十二指肠、空肠和结肠植入应力传感器记录胃肠运动,经颈外静脉和侧脑室置管供注射药物,分别观察在麻醉、术后清醒、静脉注射阿托品50μg/kg以及双侧颈迷走神经切断状态下侧脑室给予不同剂量的氢溴酸槟榔碱(1.25、2.5和5μg)对犬胃肠收缩运动的影响。结果:脑室给予不同剂量的氢溴酸槟榔碱(1.25、2.5和5μg)可剂量依赖性增强麻醉和清醒状态下犬胃体、胃窦、十二指肠、空肠、回肠和结肠的收缩运动。静脉注射阿托品和双侧颈迷走神经切断均可完全阻断氢溴酸槟榔碱对犬胃肠运动的增强作用。结论:氢溴酸槟榔碱可通过中枢神经迷走胆碱能系统促进犬胃肠运动。Objective To investigate the central nervous mechanism of arecoline hydrobromide in enhancing gastrointestinal motility.Methods The stress sensors were implanted in the stomach,antrum,duodenum,jejunum and colon of beagles to record gastrointestinal movement.Chronic venous catheters were implanted in the external jugular vein for drug injection.Lateral ventricular catheters were implanted under the dog brain stereotactic instrument for lateral ventricular drug administration.The effects of intracerebroventricular injection of arecoline hydrobromide(1.25μg,2.5μg and 5μg)were recorded under anesthesia,conscious state,atropine intravenous injection and after bilateral cervical vagotomy on gastrointestinal contractions in dogs.Results Intracerebroventricular injected arecoline hydrobromide(1.25μg,2.5μg and 5μg)could enhance the contractile movement of gastric body,gastric antrum,duodenum,jejunum,ileum and colon in both anesthetized and conscious dogs in a dose-dependent manner.Intravenous atropine 50μg/kg could completely block the enhancing effect of arecoline hydrobromide on gastrointestinal motility in dogs.The enhancement of gastrointestinal motility by intracerebroventricular injection of arecoline hydrobromide can be completely eliminated after bilateral cervical vagotomy.Conclusion The arecoline hydrobromide can promote gastrointestinal movement in dogs through central nervous vagal cholinergic system.

关 键 词：氢溴酸槟榔碱 胃肠动力 中枢神经系统 迷走神经 

分 类 号：R961[医药卫生—药理学]