高效液相色谱测定防腐木中IPBC含量的方法研究  

Method of Detecting the Content of IPBC in Preservative-Treated Wood Using High Performance Liquid Chromatography

作　　者：张景朋 张卿硕 韩利平 蒋明亮[1] ZHANG Jing-peng;ZHANG Qing-shuo;HAN Li-ping;JIANG Ming-liang(Research Institute of Wood Industry,Chinese Academy of Forestry,Beijing 100091,China)

机构地区：[1]中国林业科学研究院木材工业研究所,北京100091

出　　处：《木材科学与技术》2022年第5期71-77,共7页Chinese Journal of Wood Science and Technology

基　　金：中国林科院院所基本科研业务费专项资金项目“木材防水防霉协同作用机制及应用技术研究”(CAFYBB2019SY029)。

摘　　要：为实现防腐处理材中碘代丙炔基丁基氨基甲酸酯(3-iodo-2-propynyl butylcarbamate,IPBC)含量的精确分析,建立IPBC的高效液相色谱(HPLC)分析方法,并对比研究了超声波提取方法中提取溶剂种类、溶剂用量、提取时间等因素对防腐杉木中IPBC提取效果的影响。采用紫外检测器及C18反相色谱柱,优化得到分析IPBC的色谱条件:V_(乙腈)∶V_(水)=52∶48为流动相,流速1.0 mL/min,检测波长200 nm。IPBC在5~120 mg/L范围内,标准曲线决定系数为0.9998,具有显著线性相关;分析样品的变异系数0.66%,样品的回收率为99.5%~100.6%。超声波提取法中,在优化条件:V_(甲醇)∶V_(水)=7∶3、液料比20 mL/g、提取时间30 min,4种材料中IPBC的回收率为97.5%~106.4%,标准偏差在0.3%~2.6%。本方法可用于检测处理材中IPBC的含量,前处理程序简便快捷,准确度高,重现性好。In order to analyze the content of 3-iodo-2-propynyl butyl carbamate (IPBC) in treated wood accurately,high performance liquid chromatography (HPLC) analytical method was established.Meanwhile,the optimization of ultrasonic extraction method on extracting IPBC from Chinese fir was conducted by comparing the effects of different solvents,the amount of solvent,and the extraction time.UV detector and C18 reversed-phase column were used for detection.The chromatographic conditions for analysis of IPBC were as follows:flow phase acetonitrile-water of 52∶48,the flow rate of 1.0mL/min,and the UV detection wavelength of 200 nm.There was a good linear relationship between IPBC concentration and chromatographic peak area in the range of 5~120 mg/L,with coefficient of determination was 0.9998.The coefficient of variation and recovery of samples were 0.66%and 99.5%~100.6%,respectively.In the ultrasonic extraction method,either methanol or methanol-water as the solvent has a better extraction effect.The methanol-water volume ratio of 7∶3 was the most optimal in minimizing the use of methanol.The amount of solvent and extraction time have a certain influence on the results,and the optimal conditions were at a ratio of 20 mL/g liquid-solid with extraction time of 30 mins.Under optimal conditions,four samples were processed and analyzed.The recovery rate of IPBC ranged between 97.5%~106.4%,with the standard deviation was 0.3%~2.6%.This method can be used for analyzing the content of IPBC in treated wood materials,not only the pre-treatment procedure is simple and quick,but also with high accuracy and good reproducibility.

关 键 词：IPBC 高效液相色谱 防腐木 超声波 提取方法 

分 类 号：S782.33[农业科学—木材科学与技术]