响应面法优化猕猴桃类胡萝卜素提取工艺  

Optimization of carotenoid extraction process of Kiwifruit by response surface methodology

作　　者：李灵[1] 熊芸 张钰瑶 杜奎 李明章[2] 高平[1] 谢玥[2] LI Ling;XIONG Yun;ZHANG Yu-Yao;DU Kui;LI Ming-Zhang;GAO Ping;XIE Yue(Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education,college of Life Sciences,Sichuan University,Chengdu 610065,China;China-New Zealand Belt and Road Joint Laboratory on Kiwifruit,Kiwifruit Breeding and Utilization Key Laboratory of Sichuan Province,Sichuan Provincial Academy of Natural Resources,Chengdu 610015,China)

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610065 [2]四川省自然资源科学研究院猕猴桃育种及利用四川省重点实验室中国-新西兰猕猴桃“一带一路”联合实验室,成都610015

出　　处：《四川大学学报（自然科学版）》2022年第5期156-162,共7页Journal of Sichuan University(Natural Science Edition)

基　　金：四川省科技计划重点研发项目(2021YFYZ0010);四川省科技计划应用基础研究(2020YJ0248)。

摘　　要：为建立与优化猕猴桃类胡萝卜素的提取方法,本文以黄肉猕猴桃‘金什1号’果肉为试验材料,利用超声辅助法提取猕猴桃果肉中的类胡萝卜素,结合单因素试验,将OD值作为检测波长,通过响应面法优化猕猴桃果肉中类胡萝卜素的提取条件.结果显示其最佳提取工艺参数为:提取溶剂乙醇-丙酮混合溶剂(体积分数=2∶1),提取时间33 min,提取温度55℃,液料比16∶1(mL∶g).根据优化条件进行试验后,猕猴桃中类胡萝卜素的提取含量为70.04䥺SymbolqB@0.66(μg/g).该工艺简便快捷、稳定可靠,可用于猕猴桃中类胡萝卜素的提取.To establish and optimize the extraction method of carotenoids from Kiwifruit, carotenoids were extracted from the flesh of yellow kiwifruit Jinshi 1’ by ultrasound-assisted extraction method. Combined with a single factor test, the ODvalue was used as the detection wavelength to optimize the extraction conditionsof carotenoids in kiwifruit flesh by response surface methodology. The results showed that the optimal extraction process parameters of carotenoid from kiwifruit were as follows: extraction solvent ethanol-acetone mixed solvent(volume fraction: 2∶1), extraction time: 33 min, extraction temperature: 55 ℃, liquid/solid ratio: 16∶1(mL∶g). Under these conditions, the extraction content of carotenoids from kiwifruit was 70.04SymbolqB@0.66(μg/g). The process is simple, fast, stable and reliable, and can be used for the extraction of carotenoids from kiwifruit.

关 键 词：猕猴桃 类胡萝卜素 响应面 提取 

分 类 号：S663.9[农业科学—果树学]