低氧对小鼠海马神经元发育相关分子表达的影响  

The effects of hypoxia on the expression of moleculas related to neuronal development in murine hippocampal neurons

作　　者：周杨 曹子鹏 陈筱鸣 李鸣 战金龙[2] 张文斌 Zhou Yang;Cao Zipeng;Chen Xiaoming;Li Ming;Zhan Jinlong;Zhang Wenbin(Department of OCcupational and Environmental Health,Air Force Medical University,Xian 710032;Medical Company of Unit 32127 of PLA,Dalian 116600,China)

机构地区：[1]空军军医大学军事预防医学系军队劳动与环境卫生学教研室,西安710032 [2]中国人民解放军32127部队卫生队,大连116600

出　　处：《神经解剖学杂志》2022年第4期417-422,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81730053)。

摘　　要：目的:探讨低氧暴露对小鼠海马神经元发育相关蛋白表达的影响,为低氧导致认知功能障碍机制的研究提供新的靶分子。方法:低氧处理小鼠海马神经元来源的HT22细胞系24 h和48 h,收集细胞样本进行转录组测序筛查低氧易感分子,real time RT-PCR检测HT22细胞中低氧相关分子纤维连接蛋白1(Fn1)、细丝蛋白C(FLNC)和细胞周期蛋白F(CCNF)mRNA的表达水平,Western Blot检测HT22细胞中Fn1、FLNC和CCNF蛋白的表达水平。结果:转录组测序筛选出低氧相关分子,低氧暴露后HT22细胞中Fn1和FLNC mRNA和蛋白水平显著降低。结论:低氧暴露可导致HT22细胞中Fn1和FLNC表达发生改变,可能是低氧诱导神经元损伤的靶分子。Objective:To investigate the effect of hypoxic exposure on the expression of development-related moleculas in HT22 murine hippocampal neuronal cells,providing new targets for the study of the mechanism of hypoxia-induced cognitive dysfunction.Methods:HT22 cells derived from mouse hippocampal neurons were treated with hypoxia for 24 and 48 h,and cell samples were collected for transcriptome sequencing to screen for hypoxia-susceptible molecules.The mRNA expression levels of hypoxia-related molecules on fibronectin 1(Fn1),filamin C(FLNC)and cyclin F(CCNF)in HT22 cells were detected by real time RT-PCR,and the corresponding proteins expression in HT22 cells were verified by Western Blot.Results:Hypoxia-related molecules were screened by transcriptomics,and the mRNA and protein levels of Fn1 and FLNC in hippocampal neurons of mice were significantly decreased after hypoxia exposure.Conclusion:Hypoxia can lead to changes in the expression of Fn1 and FLNC in HT22 cells,which may be target molecules of hypoxia-induced neuronal damage.

关 键 词：低氧环境 认知损伤 神经元发育 纤维连接蛋白1 细丝蛋白C HT22细胞 

分 类 号：R338[医药卫生—人体生理学]