马铃薯帚顶病毒基因组克隆与侵染性克隆构建  

Cloning of Potato Mop-top Virus Genome and Construction of Infectious cDNA Clone

作　　者：刘野 高艳玲[2] 刘佳慧 方月 马廷帅 毛彦芝[2] 张丽莉[1] 白艳菊[2] 程晓非 LIU Ye;GAO Yanling;LIU Jiahui;FANG Yue;MA Tingshuai;MAO Yanzhi;ZHANG Lili;BAI Yanju;CHENG Xiaofei(Key Laboratory of Germplasm Innovation and Physiology and Ecology of Food Crops in Cold Regions,Ministry of Education/College of Agronomy,Northeast Agricultural University,Harbin,Heilongjiang 150030,China;Industrial Crops Institute,Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086,China)

机构地区：[1]寒地粮食作物种质创新与生理生态教育部重点实验室/东北农业大学农学院,黑龙江哈尔滨150030 [2]黑龙江省农业科学院经济作物研究所,黑龙江哈尔滨150086

出　　处：《中国马铃薯》2022年第4期322-331,共10页Chinese Potato Journal

基　　金：国家自然科学基金资助项目(31671998);黑龙江省自然科学基金重点项目(ZD2018002)。

摘　　要：马铃薯帚顶病毒(Potato mop-top virus,PMTV)能严重影响马铃薯(Solanum tuberosum)的产量和品质,是中国进境植物一类检疫性有害生物。中国广东、云南、四川等地马铃薯上曾零星检测到该病毒的存在。文中报道了一株采自河北省PMTV马铃薯分离物的全基因组序列,分析了该分离物与其他PMTV分离物的遗传进化关系。通过酵母同源重组法构建了该分离物的侵染性克隆,并在本氏烟(Nicotiana benthamiana)和马铃薯上验证了该分离物的侵染性。研究丰富了对侵入中国的PMTV的了解,为进一步开展PMTV的致病机理、马铃薯抗病机制以及粉痂菌(Spongospora subterranea)传毒机制奠定了基础。Potato mop-top virus(PMTV) can seriously affect the yield and quality of potato(Solanum tuberosum)and it is included in the list of quarantine pests of imported plants of the People’s Republic of China. PMTV has been sporadically detected on potatoes from Guangdong, Yunnan, and Sichuan provinces in China. The full genome sequence isolated from a PMTV potato tuber collected in Hebei Province was reported and the genetic evolutionary relationships of this PMTV isolate and other ones were analyzed. The infectious clone of this PMTV isolate was constructed with the yeast homologous recombination method and its infectivity was verified on Nicotiana benthamiana and potato. This study would increase the knowledge about the invasion of PMTV to China and lay the foundation for studying the pathogenesis of PMTV, the resistance mechanism of potatoes, and the transmission mechanism of powdery mildew(Spongospora subterranea).

关 键 词：马铃薯帚顶病毒 侵染性克隆 全基因组 河北分离物 序列分析 

分 类 号：S532[农业科学—作物学]