热休克蛋白90α在活动性肺结核患者血液中的表达研究  

Study on the expression of heat shock protein 90αin the blood of patients with active pulmonary tuberculosis

作　　者：李烨 刘佳星 岳莉[1] 查晓丹 戚应杰[1] LI Ye;LIU Jiaxing;YUE Li;ZHA Xiaodan;QI Yingjie(Clinical Labortory,Anhui Provincial Hospital Infection Hospital,Hefei,Anhui 230000,China)

机构地区：[1]安徽省立医院感染病院检验科,安徽合肥230000

出　　处：《大医生》2022年第20期13-16,共4页Doctor

摘　　要：目的探讨热休克蛋白90α(heat shock protein,Hsp90α)在活动性肺结核(active tuberculosis,ATB)患者血液中的表达情况并分析其对区分活动性肺结核和潜伏性肺结核的诊断价值。方法选取2021年1月至6月安徽省立医院感染病院已确诊的81例ATB患者和65例潜伏性肺结核(latent tuberculosis infection,LTBI)患者,分别记为活动组和潜伏组,观察比较两组患者血浆标本中Hsp90α的表达情况,采用接受者操作特征(receiver operating characteristic,ROC)曲线分析Hsp90α和T细胞酶联免疫斑点试验(T-spot.TB)两种检测技术诊断ATB的效能(敏感度和特异度)。结果ATB组的Hsp90α水平显著高于LTBI组,差异有统计学意义(P<0.05),其区分ATB和LTBI的敏感度和特异度分别为74.1%和81.8%,ROC曲线下面积为0.745(95%CI:66.4%~82.6%);T-spot.TB用于区分ATB和LTBI的敏感度和特异度分别为40.7%和84.4%,ROC曲线下面积为0.624(95%CI:0.536~0.712)。结论当结核分枝杆菌感染后,会造成血浆中Hsp90α表达的上调,且在ATB的阳性率和表达水平高于LTBI,Hsp90α定量检测在区分ATB和LTBI患者上具有较高的敏感度和特异度,且其敏感度要远高于T-spot.TB。Hsp90α有望成为辅助诊断活动性肺结核的一种新的生物标志物选项。Objective We reported that the expression of heat shock protein 90α(Hsp90α)in the blood of patients with active pulmonary tuberculosis(ATB)and analyze the diagnostic significance in distinguishing active pulmonary tuberculosis from latent pulmonary tuberculosis.Methods In this study,81 patients with ATB and 65 patients with latent tuberculosis(LTBI)diagnosed in the pulmonary tuberculosis area of Anhui Provincial Hospital Infection Hospital from January 2021 to June 2021 were selected to observe and compare the plasma samples of the two groups of patients.The Hsp90αexpression at ATB has been observed,the receiver operating characteristic(ROC)was used to analyze the sensitivity and specificity of the diagnosis of ATB,and the diagnostic efficacy(sensitivity and specificity)of Hsp90αand T-cell enzyme-linked immunospot test(T-spot.TB)was compared.Results Hsp90αin plasma was over represented at the ATB group and higher than that in the LTBI group,with statistically significant difference(P<0.05).Its sensitivity and specificity for differentiating ATB and LTBI were 74.1%and 81.8%raspectively;The area under the ROC curve was 0.745(95%CI:66.4%to 82.6%).The susceptibility and atopic of T-spot.TB for distinguishing active and latent tuberculosis were 40.7%and 84.4%,and the area under the ROC curve was 0.624(95%CI:0.536~0.712).Conclusion After Mycobacterium tuberculosis infection,Hsp90αin plasma will be up-regulated,and the positive rate and expression level of ATB are higher than those of LTBI.The sensitivity and specificity of quantitative detection of Hsp90αare higher than T-spot.TB.in diagnasing ATB.Hsp90αis expected to be a new biomarker option to aid in the diagnosis of ATB.

关 键 词：热休克蛋白90Α 活动性肺结核 潜伏性肺结核 T细胞酶联免疫斑点试验 

分 类 号：R521[医药卫生—内科学]