Nano-hydroxyapatite(n-HA)involved in the regeneration of rat nerve injury triggered by overloading stretch  

作　　者：Meili Liu Chongquan Huang Zhijun Zhao Anqing Wang Ping Li Yubo Fan Gang Zhou 

机构地区：[1]Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education,School of Biological Science and Medical Engineering,Beihang University,Beijing,100083,China [2]Beijing Advanced Innovation Centre for Biomedical Engineering,Beihang University,Beijing,102402,China [3]Shenzhen Research Insititute of Beihang University,Beihang University,Shenzhen,518057,China [4]National Research Center for Rehabilitation Technical Aids,Beijing,100176,China

出　　处：《Medicine in Novel Technology and Devices》2019年第4期24-30,共7页医学中新技术与新装备（英文）

基　　金：supported by funds from the National Natural Science Foundation of China(NSFC)Research Grant(31971238,51574246,61871014,11827803,31771019);National Key Research and Development Program(2016YFC1100704,2016YFC1102203);also supported by 111 Project(B13003);International Joint Research Center of Aerospace Biotechnology and Medical Engineering,Ministry of Science and Technology,China.

摘　　要：Damage of axon and glial scars formation both inhibit nerve regenerative growth during nerve injury.In addition,mechanical stretch at high displacement rates of 10%tensile strain can cause marked nerve injury,it is important for finding a proper nano biomaterial to repair nerve injury.Nano-hydroxyapatite(n-HA)has excellent biocompatibility and high bioactivity,which is a good candidate for biomedical engineering applications.But the certain mechanism of n-HA on the injured nerve is seldom reported.In this study,we determined the role of n-HA on the mechanical stretch-induced nerve injury at adult rat spine.Mechanical stretch under strain 10%at displacement rates of 60 mm/min can cause marked broken vessels and edema in spinal cord and dorsal root ganglion tissue in haematoxylin-eosin(HE)staining.However,n-HA application can reverse hemorrhage and edema triggered by high rates of 60 mm/min stretch.Moreover,n-HA can promote positive staining of Netrin-1 increase significantly in spinal cord and dorsal root ganglion tested by immunohistochemistry(IHC)staining.In general,our study indicated that n-HA can repair mechanical stretch-induced nerve injury,it may provide a new approach to block injury and accelerate nerve regeneration in future.

关 键 词：Nano-hydroxyapatite(n-HA) Mechanical stretch Nerve injury NETRIN-1 Nerve regeneration 

分 类 号：R65[医药卫生—外科学]