三类抗蝮蛇毒血清的聚丙烯酰胺凝胶电泳比较研究  

A COMPARATIVE STUDY OF POLYACRYLAMIDE ELECTROPHORESIS FROM THREE KINDS OF HORSE ANTIVENINS AGAINST THE VENOM OF AGKISTRODON HALYS (PALLAS)

作　　者：谢占泰 童慧生 颜又珠 Xie Zhantai;Tong Huisheng;Yan Youzhu(Teaching Research Group of Biochemistry,East China Institute of Chemical Technology,Shanghai)

机构地区：[1]华东化工学院生物化学教研组

出　　处：《Zoological Research》1982年第4期423-425,422,共4页动物学研究（英文）

摘　　要：用聚丙烯(月先)胺凝胶电泳对三种类型的抗蝮蛇毒血清作了比较研究,结果表明,精制抗蝮蛇毒血清的凝胶电泳谱显然不同于其它两者,仅显示一条明显的抗体蛋白带,而且这种抗体蛋白与正常马血清免疫球蛋白IgG相等。Three kinds of horse anti-sera against the venom of Agkislrodon halys (Palls), i.e. the erode whole serum, the antivenin purified by ammonium sulphate precipitation and the antivenin digested with pepsin and purified by ammonium sulphate precipitation were compared by polyacrylamide gel rlcctrophoresis. the results show that the third kind of antivenin is apparently different from the other two kinds and it shows only one obvious the antibody protein band on polyacrylamide gel electrophoretic pattern. The antibody protein corresponds to the IgG of normal horse serum.

关 键 词：抗蝮蛇毒血清 聚丙烯酰胺凝胶电泳 免疫球蛋白IGG 马血清 抗体蛋白 

分 类 号：TQ3[化学工程]