Three-dimensional silk fibroin microsphere-nanofiber scaffolds for vascular tissue engineering  

作　　者：Qiang Liu Guoliang Ying Nan Jiang Ali KYetisen Danyu Yao Xiaoying Xie Yubo Fan Haifeng Liu 

机构地区：[1]Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education,School of Biological Science and Medical Engineering,Beijing Advanced Innovation Centre for Biomedical Engineering,Beihang University,Beijing,100191,People’s Republic of China [2]Department of Chemistry,Stanford University,CA,94305,USA [3]School of Materials Science and Engineering,Wuhan Institute of Technology,Wuhan,430205,People’s Republic of China [4]Division of Engineering in Medicine,Department of Medicine,Brigham and Women’s Hospital,Harvard Medical School,Cambridge,MA,02139,USA [5]School of Engineering and Applied Sciences,Harvard University,Cambridge,MA,02138,USA [6]Department of Chemical Engineering,Imperial College London,London,SW72AZ,UK [7]School of Life Information Science and Instrument Engineering,Hangzhou Dianzi University,Hangzhou,310018,Zhejiang Province,People’s Republic of China

出　　处：《Medicine in Novel Technology and Devices》2021年第1期52-59,共8页医学中新技术与新装备（英文）

基　　金：This work was supported by the National Natural Science Foundation of China(31771058,32071359,11421202,61227902,and 11120101001);National Key Technology R&D Program(2016YFC1100704,2016YFC1101101);International Joint Research Center of Aerospace Biotechnology and Medical Engineering from Ministry of Science and Technology of China,111 Project(B13003);Research Fund for the Doctoral Program of Higher Education of China(20131102130004);Fundamental Research Funds for the Central Universities.G.L.Y.thanks Hubei Provincial Natural Science Foundation of China(No.2014CFB778).

摘　　要：A significant limitation in the engineering of artificial small-diameter vascular scaffolds is that the number of endothelial cells(ECs)is not sufficient to generate a confluent coverage of the vascular scaffolds,so that the surfaces of vascular scaffolds form thrombus via platelet adhesion and aggregation.Thrombus decrease relies on three-dimensional(3D)scaffolds to mimic the natural extracellular matrix(ECM)as templates to regulate cell behavior and facilitate tissue maturation.Here,we developed 3D scaffolds consisting of silk fibroin(SF)nanofibers and homogeneous microspheres by electrospinning and microfluidics.The nanofibers with diameters ranging from 250 to 350 nm doped with microspheres(2–10μm)formed bridge-shaped structures.ECs were seeded and maintained on the 3D microsphere-nanofiber scaffolds with a mean fiber diameter of 300 nm.A 10%higher ratio of cell proliferation on 3D microsphere-nanofiber SF scaffolds was noted as compared to that on microporous and sponge-like SF scaffolds with small surface network fabricated by freeze-drying.Moreover,the gene transcript levels including CD146,VE-C and PECAM-1 were better preserved on 3D microsphere-nanofiber SF scaffolds than those on freeze-dried scaffolds.Thus,the developed 3D microsphere-nanofiber structure may have a myriad of applications in vascular tissue engineering scaffolds and cardiovascular devices.

关 键 词：MICROFLUIDICS Silk fibroin ELECTROSPINNING MICRO/NANOSTRUCTURE Tissue engineering 

分 类 号：X70[环境科学与工程—环境工程]