眼镜王蛇蛇毒的柱层析分离及神经毒组份的研究  

COLUMN CHROMATOGRAPHY FRACTIONATION OF OPHIOPHAGUS HANNAH VENOM AND STUDY OF NEUROTOXIC PEAKS

作　　者：蔡景霞[1] 孙欣 杨长久[1] 陈锡兰 李朝达[1] 赵其昆[1] 田云芬 熊郁良[1] Cai Jing-xia;Sun Xin;Yang Chang-jiu;Chen Xi-lan;Li Chao-da;Zhao Qi-kun;Tian Yun-fen;Xiong Yu-liang(Kunming Institute of Zoology,Acadernia Sinica)

机构地区：[1]中国科学院昆明动物研究所

出　　处：《Zoological Research》1980年第3期319-325,共7页动物学研究（英文）

摘　　要：用羧甲基葡聚糖C—25和氯化钠直线梯度洗脱方法,对我国广西产眼镜王蛇蛇毒进行了柱层析分离,获得了17个蛋白峰,测定了17个蛋白峰的毒性和生理活性。结果表明有7个毒性峰,其中6个峰具有阻断神经—肌肉接头传递的作用,另一个峰毒性很小,在相同剂量下也不表现上述作用。We fractionated Chinese Guangxi Ophiophagus hannah venom by CM-Sephadex C-25 column chromatography. Linear gradient elution was carried out with NaC1. Seventeen protein peaks were obtained. Their toxicity and physiological activities were estimated. Of the seven toxic peaks, six had the effect of blockading the transmission of neuromuscle juncture. The other one was much less toxic. It didn’t show such effect with the same dose.

关 键 词：眼镜王蛇 蛋白峰 柱层析分离 羧甲基葡聚糖 神经毒 蛇毒 生理活性 相同剂量 

分 类 号：R28[医药卫生—中药学]