Tea and Citrus maxima complex induces apoptosis of human liver cancer cells via PI3K/AKT/mTOR pathway in vitro  

作　　者：Shuai Wen Ran An Dongli Li Junxi Cao Zhigang Li Wenji Zhang Ruohong Chen Qiuhua Li Xingfei Lai Lingli Sun Shili Sun 

机构地区：[1]Tea Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Tea Resources Innovation&Utilization,Guangzhou,510640,China [2]School of Biotechnology and Health Sciences,Wuyi University,Jiangmen 529020,China [3]International Healthcare Innovation Institute(Jiangmen),Jiangmen 529040,China

出　　处：《Chinese Herbal Medicines》2022年第3期449-458,共10页中草药（英文版）

基　　金：supported by the"14th Five-Year Plan"teambuilding projects of Guangdong Academy of Agricultural Sciences[202126TD];Guangdong Basic and Applied Basic Research Foundation[2020A1515011266,2021A1515010958];Guangzhou Science and Technology Plan Projects[202002030202,202102020047];Key-Area Research and Development Program of Guangdong Province[2020B0202080003];Innovation Fund projects of Guangdong Academy of Agricultural Sciences[202115,202035];Special fund for scientific innovation strategy-construction of high level Academy of Agriculture Science[R2019PY-JX004]

摘　　要：Objective:In this study,black tea and Citrus maxima(BT-CM),yellow tea and C.maxima(YT-CM),green tea and C.maxima(GT-CM)as subjects,the active ingredient content and antioxidant activity of three tea and C.maxima(T-CM)were analyzed.The effects of three T-CMs on apoptosis of liver cells in vitro and its mechanism were further explored.Methods:National standard method and HPLC were used for active ingredient analysis.MTT,cell fow cytometry and Western blot were used to analyze the effects of three T-CMs on cell proliferation,apoptosis,and its underlying molecular mechanism.Results:The content of tea polyphenols,free amino acids,ratio of polyphenols and amino acids,ester catechins,non-ester catechins and caffeine in YT-CM and GT-CM was significantly higher than that of BT-CM.The in vitro antioxidant capacity of YT-CM and GT-CM was also significantly stronger than that of BT-CM.Three T-CMs had the effects of inhibiting proliferation,arresting cell cycle and inducing apoptosis in HepG2 and Bel7402 cells,especially YT-CM and GT-CM.Western blot analysis showed three T-CMs activated PI3K/AKT/mTOR signaling pathway and regulated the expression levels of apoptosis-related proteins Bax,Bcl-2 and Caspase-3/9.YT-CM and GT-CM had better ability to change the signal pathway than BT-CM.Conclusion:In short,T-CMs,which combined different degrees of fermentation tea with C.maxima,were rich in nutrients and biologically active substances.T-CMs,especially YT-CM and GT-CM,are healthy drinks that help to prevent and treat liver cancer.

关 键 词：APOPTOSIS Citrus maxima cv.Tomentosa liver cancer signaling pathway TEA 

分 类 号：R285[医药卫生—中药学]