CRISPR-Cas系统在新型冠状病毒检测与治疗中的应用研究进展  

Advances in the application of CRISPR-Cas system for SARS-CoV-2 diagnostic test

作　　者：王姗姗[1] 张丽月 闫君 刘珞[2] 卢晅[1] 刘万红[1] WANG Shan-shan;ZHANG Li-yue;YAN jun;LIU Luo;LU Xuan;LIU Wan-hong(Hubei Province Key Laboratory of Allergy and Immunology,Department of Immunology,School of Basic Medical Sciences,Wuhan University,Wuhan,Hubei 430071,China;不详)

机构地区：[1]武汉大学泰康医学院(基础医学院)过敏及免疫相关疾病湖北省重点实验室,湖北武汉430071 [2]北京化工大学生命科学与技术学院,北京100029

出　　处：《中国病毒病杂志》2022年第4期304-310,共7页Chinese Journal of Viral Diseases

基　　金：国家自然科学基金面上项目(52073022)。

摘　　要：新型冠状病毒肺炎(新冠肺炎)自2019年末暴发以来,迅速形成全球性的大流行,严重威胁人民的生命与健康,深刻影响了世界的社会、经济秩序。对新型冠状病毒(新冠病毒)快速准确的诊断有助于阻止疾病的传播;虽然目前市面上已经出现了很多新冠病毒检测方法,并在一定程度上控制了对新冠肺炎的传播,但其仍然存在一定的局限性。近年来,成簇规律间隔短回文重复序列及其相关蛋白(clustered regularly interspaced short palindromic repeats-Cas,CRISPR-Cas)系统的研究越来越深入,其展现出的高特异性及高灵敏性在分子诊断及治疗方面具有巨大潜力。本文总结了CRISPR-Cas系统的发展及其在新冠病毒检测与治疗中的应用,并对其在临床转化上面临的挑战及未来发展进行展望,以期为控制新冠肺炎疫情的流行提供参考和帮助。The outbreak of coronavirus disease 2019(COVID-19),caused by severe acute respiratory syndrome coronavirus(SARS-CoV-2)at the end of 2019,results in a global rapid pandemic.The emerging infectious disease is life threaten and profoundly undermines the normal operation all over the world.Rapid and accurate detection of SARS-CoV-2 is an essential component of efforts to combat SARS-CoV-2 spread.Although many technologies for SARS-CoV-2 detection have been commercialized and have played a role in the control of COVID-19 epidemic to some extent yet,each of them is still with certain limitations.Recently,increasing number of teams attempt to detect SARS-CoV-2 by CRISPR-Cas(clustered regularly interspaced short palindromic repeats-Cas)system.With excellent specificity and sensitivity,it has been believed to be a potential technology in COVID-19 diagnosis and therapy.The review provides an overview of CRISPR-Cas system for SARS-CoV-2 detection and COVID-19 therapy,along with its clinical translation potential.Hope it has some referential significance for the control of SARS-CoV-2 spread and COVID-19 epidemic.

关 键 词：新型冠状病毒肺炎 新型冠状病毒 CRISPR-Cas 核酸检测 疾病治疗 研究进展 

分 类 号：R183.3[医药卫生—流行病学]