半夏不同炮制品刺激性毒性变化及凝集素蛋白检测研究  

Irritant toxicity and lectin content of different processed products of Pinelliae Rhizoma

作　　者：程砚秋 郁红礼[1,2,3] 吴皓[1,2,3] 陶兴宝 谢雨薇 陈盛君[4] 张萍[5] 李松[4] 王彩霞 王贺鹏 曾平 刘冰冰 CHENG Yan-qiu;YU Hong-li;WU Hao;TAO Xing-bao;XIE Yu-wei;CHEN Sheng-jun;ZHANG Ping;LI Song;WANG Cai-xia;WANG He-peng;ZENG Ping;LIU Bing-bing(Pharmacology College,Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Key Laboratory of Chinese Medicine Processing,Nanjing 210023,China;Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing,Nanjing 210023,China;Jiangyin Tianjang Pharmaceutical Co.,Ld.,Jiangyin 214400,China;National Institutes for Food and Drug Control,Bejing 100050,China)

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]江苏省中药炮制重点实验室,江苏南京210023 [3]国家教育部中药炮制规范化及标准化工程研究中心,江苏南京210023 [4]江阴天江药业有限公司,江苏江阴214400 [5]中国食品药品检定研究院,北京100050

出　　处：《中国中药杂志》2022年第17期4627-4633,共7页China Journal of Chinese Materia Medica

基　　金：国家重点研发计划项目(2018YFC1707000);国家自然科学基金项目(81573605)。

摘　　要：为研究半夏不同炮制品炮制过程中刺激性毒性与凝集素蛋白含量变化的相关性,考察Western blot法作为凝集素蛋白检查方法的可行性。自制不同炮制程度的清半夏、法半夏和姜半夏,采用家兔眼结膜刺激模型考察不同炮制程度的半夏炮制品的刺激性毒性变化,采用Western blot法研究半夏不同炮制品中凝集素蛋白的含量变化,分析炮制过程中刺激性毒性变化与凝集素蛋白含量变化的相关性,收集多批次半夏不同炮制品饮片,检测其中的凝集素蛋白含量。结果显示,随着炮制时间的延长,3种半夏炮制品刺激性毒性逐渐降低,凝集素蛋白含量逐渐下降。清半夏浸泡4 d、法半夏浸泡2 d及姜半夏煮制0.5 h后刺激性毒性显著降低,凝集素蛋白含量显著下降。炮制过程中凝集素蛋白含量下降趋势与刺激性毒性变化趋势一致,表明凝集素蛋白含量变化能够反映刺激性毒性的变化。在清半夏、法半夏及姜半夏供试品免疫印迹图中,均未检出活性凝集素蛋白条带,表明以Western blot法检测凝集素蛋白可作为半夏炮制品中毒性凝集素蛋白检测方法。半夏按药典方法炮制合格后不应检测出凝集素蛋白。The present study aims to investigate the correlation between irritant toxicity variation and lectin content variation during the processing of Pinelliae Rhizoma products and to explore the feasibility of Western blot as a method for the detection of lectin. We processed Pinelliae Rhizoma Praeparatum Cum Alumine, Pinelliae Rhizoma Praeparatum, and Pinelliae Rhizoma Praeparatumcum Zingibere et Alumine to different degrees and then analyzed their irritant toxicity via Draize rabbit eye test. Western blot was employed to determine the lectin content in Pinelliae Rhizoma products processed with different methods. The correlation between toxicity variation and lectin content variation was then analyzed. Different decoction pieces of Pinelliae Rhizoma were collected for the determination of lectin content. The three processed products of Pinelliae Rhizoma showed gradually decreased toxicity and lectin content as the processing continued. The decreasing trend of lectin content was consistent with that of irritant toxicity during processing, which indicated that the change in lectin content could reflect the trend of irritant toxicity. No band of lectin appeared in the Western blot of processed products of Pinelliae Rhizoma, which suggested that western blotting can be used for the detection of toxic lectin in the processed products of Pinelliae Rhizoma. Lectin should not be detected in the Pinelliae Rhizoma products processed according to the methods in the Chinese Pharmacopoeia.

关 键 词：半夏 炮制 刺激性毒性 凝集素蛋白检测 

分 类 号：R283.1[医药卫生—中药学]