Global Quantitative Proteomics Analysis Reveals the Downstream Signaling Networks of Msx1 and Msx2 in Myoblast Differentiation  

作　　者：Guoqiang Zhou Shuangping Ma Ming Yang Yenan Yang 

机构地区：[1]State Key Laboratory of Genetic Engineering,School of Life Sciences,Fudan University,Shanghai 200438,China

出　　处：《Phenomics》2022年第3期201-210,共10页表型组学（英文）

基　　金：This work was supported by the grant from the National Natural Science Foundation Grant of China to Jingqiang Wang(31972885).

摘　　要：The msh homeobox 1(Msx1)and msh homeobox 2(Msx2)coordinate in myoblast differentiation and also contribute to muscle defects if altered during development.Deciphering the downstream signaling networks of Msx1 and Msx2 in myoblast differentiation will help us to understand the molecular events that contribute to muscle defects.Here,the proteomics characteristics in Msx1-and Msx2-mediated myoblast differentiation was evaluated using isobaric tags for the relative and absolute quantification labeling technique(iTRAQ).The downstream regulatory proteins of Msx1-and Msx2-mediated differentiation were identified.Bioinformatics analysis revealed that these proteins were primarily associated with xenobiotic metabolism by cytochrome P450,fatty acid degradation,glycolysis/gluconeogenesis,arginine and proline metabolism,and apoptosis.In addition,our data show Acta1 was probably a core of the downstream regulatory networks of Msx1 and Msx2 in myoblast differentiation.

关 键 词：Quantitative proteomics MSX1 Msx2 Myoblast differentiation 

分 类 号：Q254[生物学—细胞生物学]