代谢流分析技术在葡萄糖代谢通路研究中的进展  

Research progress of metabolic flux analysis technology in glucose metabolism pathway research

作　　者：赵云昊 令狐婷 武文泽 王贤贤 韩雨梅[2] 秦雪梅[1] 田俊生[1] ZHAO Yun-hao;LINGHU Ting;WU Wen-ze;WANG Xian-xian;HAN Yu-mei;QIN Xue-mei;TIAN Jun-sheng(Modern Research Center for Traditional Chinese Medicine,Shanxi University,Taiyuan 030006,China;School of Physical Education,Shanxi University,Taiyuan 030006,China)

机构地区：[1]山西大学中医药现代研究中心,山西太原030006 [2]山西大学体育学院,山西太原030006

出　　处：《药学学报》2022年第9期2731-2737,共7页Acta Pharmaceutica Sinica

基　　金：国家“重大新药创制”科技重大专项资助项目(2017ZX09301047);国家自然科学基金资助项目(82074147);山西省科技重点研发计划资助项目(201903D321210);山西省“1331工程”协同创新中心建设计划;山西省应用基础研究项目(201801D121291);山西省基础研究计划(202103021224026).

摘　　要：随着稳定同位素示踪代谢组学技术的广泛应用,对数据的全面分析和深入挖掘显得尤为重要,代谢流分析是主要的技术手段之一,特别是在葡萄糖代谢研究中受到高度关注。代谢流分析技术将同位素示踪与数学模型结合,推导计算出代谢物间的代谢通量,为研究提供了更多的信息,更清晰、具体地反映了一个动态的代谢过程。本文对代谢流分析在葡萄糖代谢研究中的基本流程、注意事项、应用实例等进行综述,为基于稳定同位素示踪代谢组学的代谢流分析技术研究葡萄糖代谢提供科学依据。With the wide application of stable isotope tracer metabolomics technology,its comprehensive analysis and in-depth mining of data are particularly important,and metabolic flux analysis is one of the main technical means,especially in the study of glucose metabolism.Metabolic flux analysis technology combines isotope tracing with mathematical models to deduce and calculate the metabolic flux between metabolites.The metabolic flux provides more information for research and reflects a dynamic metabolic process more clearly and specifically.This paper reviews the basic process,precautions,and application examples of metabolic flux analysis in glucose metabolism research,and provides a reference for the application of metabolic flux analysis based on stable isotope tracer metabolomics in glucose metabolism research.

关 键 词：代谢流分析 稳定同位素示踪代谢组学 葡萄糖代谢 代谢通路 

分 类 号：R917[医药卫生—药物分析学]