纤枝金丝桃中的一个新的多环多异戊烯基间苯三酚衍生物  

A new polycyclic polyprenylated acylphloroglucinols from Hypericum lagarocladum N.Robson

作　　者：邓憬童 周童曦 韩庆迪 程海涛 刘阳[2] 王强[1] 杨新洲[1] DENG Jing-tong;ZHOU Tong-xi;HAN Qing-di;CHENG Hai-tao;LIU Yang;WANG Qiang;YANG Xin-zhou(School of Pharmaceutical Sciences,South-Central University for Nationalities,Wuhan 430074,China;College of Life Science,Wuchang University of Technology,Wuhan 430223,China)

机构地区：[1]中南民族大学药学院,湖北武汉430074 [2]武昌理工学院生命科学学院,湖北武汉430223

出　　处：《药学学报》2022年第9期2767-2773,共7页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金项目(81774000,81573561);湖北省卫健委中医药科研项目(ZY2021M076).

摘　　要：采用硅胶柱色谱、反相C18色谱柱色谱、Sephadex LH-20凝胶柱色谱、高效液相色谱及中压半制备液相色谱等分离技术对纤枝金丝桃中的化学成分进行分离纯化,通过MS、NMR等波谱数据结合理化性质鉴定化合物结构。从纤枝金丝桃80%乙醇提取物中分离得到11个化合物,分别鉴定为hyperlagarone A(1)、hyperpatulone E(2)、hyperbeanol G(3)、uralione D(4)、tomoeone F(5)、pyramidatone A(6)、tomoeone A(7)、tomoeone B(8)、hyperbeanol C(9)、hyperbeanol A(10)、hypercohone G(11)。其中化合物1是未见报道的新化合物,化合物2~11是首次从该植物中分离得到。对11个化合物进行基于L6骨骼细胞的促葡萄糖摄取实验,结果显示化合物7、8均显示较强的促葡萄糖摄取活性。Silica gel column chromatography,reversed phase C18 column chromatography,Sephadex LH-20 gel column chromatography,high performance liquid chromatography and medium performance semi preparative liquid chromatography were performed to separate and purify the chemical constituents of Hypericum lagarocladum N.Robson.Spectroscopic methods such as MS and NMR combined with physicochemical properties were applied in identifying the structures of the isolated compounds.A total of 11 compounds were isolated and identified as hyperlagarone A(1),hyperpatulone E(2),hyperbeanol G(3),uralione D(4),tomoeone F(5),pyramidatone A(6),tomoeone A(7),tomoeone B(8),hyperbeanol C(9),hyperbeanol A(10),and hypercohone G(11),respectively.Compound 1 is a new polycyclic polyprenylated acylphloroglucinol derivative,and compounds 2-11 are isolated from this plant for the first time.11 compounds were tested for glucose uptake in L6 cells,and the results showed that compounds 7 and 8 had significant effect on glucose uptake.

关 键 词：藤黄科 纤枝金丝桃 化学成分 多环多异戊烯基间苯三酚 葡萄糖摄取 

分 类 号：R284[医药卫生—中药学]