基于UHPLC-LTQ-orbitrap-MS方法分析藏药洪连(短管兔耳草)中黄酮与苯乙醇苷类成分  

Analysis of flavonoids and phenylethanoid glycosides in the Tibetan herb Lagotis brevituba Maxim based on UHPLC-LTQ-orbitrap-MS

作　　者：耿昭 高必兴 钟恋 齐景梁[2] 苟琰[1] 蒋运斌 杨蕾 袁军 郭力 王一涛[3] GENG Zhao;GAO Bi-xing;ZHONG Lian;QI Jing-liang;GOU Yan;JIANG Yun-bin;YANG Lei;YUAN Jun;GUO Li;WANG Yi-tao(NMPA Key Laboratory for Quality Evaluation of Traditional Chinese Medicine(Traditional Chinese Patent Medicine),Sichuan Institute for Drug Control(Sichuan Testing Center of Medical Devices),Chengdu 611731,China;College of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;State Key Laboratory of Quality Research in Chinese Medicine,Institute of Chinese Medical Sciences,University of Macao,Macao 999078,China;College of Pharmaceutical Sciences,Southwest University,Chongqing 400715,China)

机构地区：[1]四川省药品检验研究院/国家药品监督管理局中成药质量评价重点实验室,四川成都611731 [2]成都中医药大学药学院,四川成都611137 [3]澳门大学中华医药研究院中药质量研究国家重点实验室,中国澳门999078 [4]西南大学药学院·中医药学院,重庆400715

出　　处：《药学学报》2022年第9期2821-2838,共18页Acta Pharmaceutica Sinica

基　　金：国家药品监督管理局注册司专项“特色民族药材检验方法研究”三期;四川省药品监督管理局科研计划项目;重庆市自然科学基金面上项目(cstc2020jcyj-msxmX0146).

摘　　要：采用超高效液相色谱-串联线性离子阱-轨道阱组合高分辨质谱(UHPLC-LTQ-orbitrap-MS)技术对藏药洪连(中国药典收载基原短管兔耳草)中黄酮类和苯乙醇苷类成分进行定性分析。采用与动态排除相结合的数据依赖性扫描,在电喷雾离子源正、负离子模式下分别分析黄酮类和苯乙醇苷类成分的精确分子量、碎片离子及保留时间等信息,并结合相关文献系统全面地鉴别藏药洪连中这两类成分。最终共鉴定了167个化合物,其中黄酮类成分84个,苯乙醇苷类成分83个,极大地丰富了洪连及兔耳草属植物中黄酮和苯乙醇苷类成分的数量和种类,特别是首次从兔耳草属植物中发现了黄酮化合物宝藿苷I、脱氧己糖与葡萄糖醛酸组合的二糖氧苷黄酮4个、碳苷黄酮9个、四糖苯乙醇苷15个以及β位有取代基的苯乙醇苷5个,为阐明洪连物质基础并整体提升其质量控制水平以及进一步规范相关基原品种的临床应用提供数据基础和科学依据。Ultra high performance liquid chromatography tandem linear ion trap orbitrap mass spectrometry(UHPLC-LTQ-orbitrap-MS)was applied to analyze and identify flavonoids and phenylethanoid glycosides in the Tibetan herb Lagotis brevituba Maxim.A method of data-dependent scan coupling with dynamic exclusion was developed for analyzing flavonoids and phenylethanoid glycosides under positive and negative ion mode of electro‐spray ionization(ESI).The compounds of Lagotis brevituba Maxim.were systematically identified through exact molecular mass,fragmentation patterns,retention time and reported references.A total of 167 compounds were detected,of which 84 were flavonoids and 83 were phenylethanoid glycosides,which greatly enriched the number and types of flavonoids and phenylethanol glycosides in Lagotis genus medicinal plants.BaohuosideⅠ,4 disaccharide O-glycoside flavonoids(composed of deoxyhexose and glucuronic acid),9 C-glycoside flavonoids,15 tetrasaccharide phenylethanoid glycosides and 5 phenylethanoid glycosides with substituents on theβ-position of the phenylethyl group were identified in Lagotis genus medicinal plants for the first time.This study provides scientific support for elucidating the material basis and improving the quality control of Lagotis brevituba Maxim.

关 键 词：洪连 UHPLC-LTQ-orbitrap-MS 黄酮 苯乙醇苷 兔耳草属 

分 类 号：R917[医药卫生—药物分析学]