巨峰系葡萄主栽品种的SRAP遗传多样性分析与MCID法快速鉴定  

Genetic Diversity Analysis of Main Kyoho Grapevine Series Cultivars Based on SRAP Markers and Rapid Identification by MCID Method

作　　者：张中海[1] 张安世[1] 高登涛[2] 魏志峰[2] ZHANG Zhonghai;ZHANG Anshi;GAO Dengtao;WEI Zhifeng(School of Science,Jiaozuo Normal College,Jiaozuo 454000;Zhengzhou Fruit Research Institute,CAAS,Zheng-zhou 450009,China)

机构地区：[1]焦作师范高等专科学校理工学院,河南焦作454000 [2]中国农业科学院郑州果树研究所,郑州450009

出　　处：《东北农业科学》2022年第4期38-42,共5页Journal of Northeast Agricultural Sciences

基　　金：中国农业科学院科技创新工程专项经费项目(CAASASTIP-2015-RIP-04);国家重点研发计划项目(2018YFD0201300)。

摘　　要：为探讨巨峰系葡萄种质的遗传关系,该研究利用SRAP标记对24个巨峰系葡萄品种进行遗传多样性分析,并应用人工绘制品种鉴别示意图(MCID)法建立品种鉴定图(CID)。筛选出12对引物对24个巨峰系葡萄品种进行PCR扩增,共扩增出126个条带,其中多态性条带99条,多态性比率为78.57%。各引物观测等位基因数(Na)、有效等位基因数(Ne)、Nei's基因多样性指数(H)和Shannon's信息指数(I)的平均值分别为1.7857、1.3581、0.2176和0.3388。24个巨峰系葡萄品种的遗传相似系数(Genetic Similarity Coefficient,GS)在0.6190~0.9603,变幅为0.3413。表明24个巨峰系葡萄品种间的遗传差异较小。利用UPGMA构建24个巨峰系葡萄种质资源的聚类树状图,在遗传相似系数为0.72处可将24个巨峰系葡萄品种分为3组,第Ⅰ组有22个品种,第Ⅱ组为奥林匹亚,第Ⅲ组为黑丰。利用6对引物扩增的多态性谱带构建了24个巨峰系葡萄品种的CID图,利用此图可以找出区分任意2个品种的引物,为巨峰系葡萄品种鉴定提供科学依据。In order to explore the genetic relationship among the germplasm resources of Kyoho grapevine series,the genetic diversity of 24 cultivars was analyzed by SRAP marker,and the cultivar identification diagram(CID)was constructed by using the manual cultivar identification diagram(MCID).12 primers were screened for PCR amplifi⁃cation of 24 cultivars.A total of 126 bands were generated,of which 99 bands were polymorphic bands(78.57%).The average value of observed number of alleles(Na),effective number of alleles(Ne),Nei's gene diversity(H)and Shannon's information index(I)were 1.7857,1.3581,0.2176 and 0.3388,receptively.The genetic similarity coeffi⁃cient among 24 cultivars ranged from 0.6190 to 0.9603,with a range of 0.3413.The above results indicate that the genetic differences among the tested cultivars were small.The dendrogram of 24 cultivars was constructed using UPGMA.They can be divided into 3 groups at genetic similarity coefficient of 0.72,of which there were 22 cultivars in Group I,while Group II was Olympia and Group III was Heifeng.CID of 24 cultivars was constructed using poly⁃morphic bands by 6 primers,by using CID we can find the primers that distinguish any two cultivars,providing sci⁃entific basis for the identification of Kyoho grapevine series.

关 键 词：葡萄 品种 SRAP 遗传多样性 人工绘制植物品种鉴别图(MCID)法 

分 类 号：S663.1[农业科学—果树学] Q943[农业科学—园艺学]