平菇球形病毒外壳蛋白基因的克隆及病毒致病性的初步分析  

Cloning of Coat Protein Gene of Oyster Mushroom Spherical Virus and Pre⁃liminary Analysis of Viral Pathogenicity

作　　者：李硕[1] 戴坚 张艳敬 李玉[1,2] LI Shuo;DAI Jian;ZHANG Yanjing;LI Yu(Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural University,Changchun 130118;College of Plant Protection,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学食药用菌教育部工程研究中心,长春130118 [2]吉林农业大学植物保护学院,长春130118

出　　处：《东北农业科学》2022年第4期43-47,共5页Journal of Northeast Agricultural Sciences

基　　金：111引智基地项目(D17014);中国博士后科学基金项目(2017M621223);吉林农业大学省级大学生创新创业训练计划项目(2017410)。

摘　　要：平菇是我国栽培面积最广的食用菌之一,在菌种繁育过程中经常会出现菌种退化、产量降低等问题,对平菇产业造成巨大经济损失,其原因之一是由病毒病害引起的。本试验采集了16株疑似平菇病毒病样品,分别选用特异扩增平菇球形病毒(Oyster mushroom spherical virus,OMSV)和平菇等轴病毒(Oyster mushroom isometric virus,OMIV)目标片段的引物,通过RT-PCR的方法鉴定出6株平菇样品感染了OMSV,而所有菌株中均未检测到OMIV。选取扩增OMSV条带最亮的6号平菇样品(Po6),克隆了OMSV外壳蛋白(coat protein,CP)基因全长片段,序列分析显示与OMSV韩国分离物(Genbank ID:NC_004560.1)CP基因的核酸序列相似度为98.18%,氨基酸序列相似度为97.89%。通过原生质体再生法对Po6菌株进行脱毒处理,发现脱毒后菌落形态更加整齐,菌丝生长速度增快,生物量增加,并且出菇试验中菇型更接近于健康菌株。这初步表明OMSV对平菇菌丝生长以及子实体发育具有抑制作用。Pleurotus ostreatus is one of the most widely cultivated edible fungi in China and has been facing some challenges,such as strain degeneration and yield reduction probably due to viral infections.Sixteen virus-like in⁃fected strains of Oyster mushroom were collected.Oyster mushroom spherical virus(OMSV)was detected in six P.ostreatus strains and Oyster mushroom isometric virus(OMIV)was not detected in all strains by RT-PCR using spe⁃cific primers.The full-length sequence of coat protein gene of OMSV in Po6 strain which has the strongest amplifi⁃cation was determined.The comparison of the coat protein sequence showed 98.18%nucleic acid identity and 97.89%amino acid identity with OMSV Korean isolate(Genbank ID:NC_004560.1).After eliminating OMSV in Po6 strain by protoplast regeneration method,the mycelia growth rate and biomass increased obviously,and the phe⁃notype was closer to the healthy ones.It indicated that OMSV probably inhibits mycelia growth and fruiting body de⁃velopment of P.ostreatus.

关 键 词：平菇球形病毒 外壳蛋白 序列测定 病毒脱毒 

分 类 号：S436.46[农业科学—农业昆虫与害虫防治]