微紫青霉F1-6对细辛叶枯病菌的抑制作用及对细辛体内防御酶活性的影响  

Inhibitory Efficiency of Penicillium janthinellum F1-6 against Asarum sieboldii Leaf Blight and Its Effect on Defensive Enzymes Activity

作　　者：刘亚苓 于营[1] 鲁海坤 雷慧霞 隋昕 郭靖[1] LIU Yaling;YU Ying;LU Haikun;LEI Huixia;SUI Xin;GUO Jing(Institute of Special Animal and Plant Sciences of CAAS,Changchun 130112,China)

机构地区：[1]中国农业科学院特产研究所,长春130112

出　　处：《东北农业科学》2022年第4期74-78,112,共6页Journal of Northeast Agricultural Sciences

基　　金：国家重点研发计划(2016YFC0500303-07);吉林省科技发展计划项目(20180201034YY);中国农业科学院科技创新工程(CAAS-ASTIP-2016-ISAPS);中央级公益性科研院所基本科研业务费专项(1610342019065)。

摘　　要：细辛叶枯病是细辛主要病害之一,发病率高,目前主要采用化学防治。本研究采用体外抑菌和盆栽试验的方法,研究微紫青霉F1-6菌株对细辛叶枯病菌的抑制效果和对健康及感病植株体内防御酶活性的影响,为细辛叶枯病的生物防治提供理论依据。结果表明,微紫青霉与叶枯病菌对峙培养后,病原菌菌落边缘菌丝出现断裂、皱缩等现象,并产生大量厚垣孢子;经微紫青霉接种处理后的健康和感病细辛叶片,其PPO、PAL、POD及SOD活性均分别显著高于未接种的健康和感病的对照组叶片。微紫青霉菌可能是通过对病原菌菌丝生长的抑制作用,以及诱导健康和感病植株防御酶活性的升高来达到控制细辛叶枯病发生和病情发展的效果。Leaf blight is one of the main diseases of Asarum sieboldii with high incidence,chemical control is mainly used.The inhibitory effect of Penicillium janthinellum F1-6 against Mycocentrospora acerina and its effect on defen⁃sive enzymes activity in vitro antifungi activity and pot experiment were studied in order to provide theoretical basis for biological control of Asarum sieboldii leaf blight.The results showed that the edge hyphae of the pathogenic fungi were broken and atrophied,and a large number of chlamydospores were produced.After inoculation,PPO,PAL,POD and SOD activities of healthy and infected Asarum sieboldii leaves were significantly higher than those of unin⁃oculated healthy and infected control leaves respectively.The research results demonstrated that Penicillium janthi⁃nellum could inhibit and control of the disease by controlling the mycelial growth of the pathogen and inducing the enzyme activity of the healthy and infected plants.

关 键 词：细辛叶枯病 微紫青霉 防御酶 生物防治 

分 类 号：R282[医药卫生—中药学]