忍冬叶生长及修剪期的7种活性成分年动态变化规律研究  

Annual dynamic variation of seven active components of Lonicera japonica in leaf growth and pruning periods

作　　者：黄厚钰 耿泽宇 李卫东[1] 张绍国 刘勇[1] HUANG Hou-yu;GENG Ze-yu;LI Wei-dong;ZHANG Shao-guo;LIU Yong(School of Chinese Materia Medica,Bejing University of Chinese Medicine,Beijing 102488,China;Bejing Jiren Medical Science and Technology Center,Bejing 100016,China)

机构地区：[1]北京中医药大学中药学院,北京102488 [2]北京济仁医药科技中心,北京100016

出　　处：《中国中药杂志》2022年第16期4341-4346,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(31370360)。

摘　　要：在生产上为促进忍冬花蕾形成,需要在忍冬生长季不断修剪,修剪下的忍冬叶资源量大,且浪费严重。该研究采用高效液相色谱(HPLC)同时检测3月—11月忍冬叶中7种活性成分(绿原酸、木犀草苷、异绿原酸A、异绿原酸B、异绿原酸C、断马钱子酸、断氧化马钱子苷)的含量。结果表明,3月忍冬树干上除去的萌蘖、5月—7月修剪下的忍冬叶、11月初霜期的忍冬叶中各活性成分含量较高,可作为忍冬非药用部位进一步开发利用。其中3月上中旬修剪下的忍冬叶中7种活性成分总含量最高。初霜后忍冬叶活性成分显著升高,接近3月上中旬修剪下的萌蘖中活性成分含量水平。初霜后的忍冬叶失去了光合能力,此时采收忍冬叶不影响树体生理活动,不但资源量巨大,而且活性成分含量高,是忍冬叶的最佳采收期。Pruning branches and leaves is the measure to stimulate the growth of Lonicera japonica flower buds, and consequently, the resources of pruned leaves are inevitably and seriously wasted in production. High-performance liquid chromatography(HPLC) was applied for content determination of seven active ingredients(chlorogenic acid, galuteolin, isochlorogenic acids A, B, and C, secologanic acid, and secoxyloganin) in L. japonica leaves from March to November. The results showed that the tillering removed from the trunk of L. japonica in March, the leaves pruned from May to July, and the leaves after the first frost date in November were rich in active ingredients, which deserved further exploitation and utilization. The total content(TC) of active ingredients in pruned L. japonica leaves in early March was the highest. The content of active ingredients in L. japonica leaves increased significantly after the first frost date, which was close to that in the bud tillers pruned in early and middle March. After the first frost date, L. japonica leaves are incapable of photosynthesis, and the harvesting of L. japonica leaves does not affect the physiological activities of the tree. In addition to huge resources, the content of active ingredients is high during this period, which is the best harvesting period of L. japonica leaves.

关 键 词：忍冬叶 活性成分 动态变化 修剪 开发利用 

分 类 号：R284.1[医药卫生—中药学]