沉香中倍半萜类化学成分研究  

Sesquiterpenoids from Aquilariae Lignum Resinatum

作　　者：张航 马家乐 昝妮利 王嵘晔 赵云芳[1] 宋月林 郑姣[1] 屠鹏飞[1] 霍会霞 李军[1] ZHANG Hang;MA Jia-le;ZAN Ni-li;WANG Rong-ye;ZHAO Yun-fang;SONG Yue-lin;ZHENG Jiao;TU Peng-fei;HUO Hui-xia;LI Jun(Modern Research Center for Traditional Chinese Medicine,School of Chinese Materia Medica,Bejing Universiy of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学中药学院中药现代研究中心,北京100029

出　　处：《中国中药杂志》2022年第16期4385-4390,共6页China Journal of Chinese Materia Medica

基　　金：国家重点研发计划“中医药现代化研究”重点专项(2018YFC1706400);国家自然科学基金项目(82003912);北京中医药大学新教师启动基金项目(2020-JYB-XJSJJ-010)。

摘　　要：采用硅胶、ODS和半制备HPLC等色谱技术对沉香石油醚部位进行化学成分研究。通过其理化性质及IR、UV、MS、NMR等谱学数据共鉴定了8个倍半萜类化合物,分别为(4S,5S,7R,10S)-5,7-dihydroxy-11-en-eudesmane(1)、(7R,10S)-eudesma-4-en-11,15-diol(2)、(2R,4S,5R,7R)-2-hydroxyeremophila-9,11-dien-8-one(3)、7α-H-9(10)-ene-11,12-epoxy-8-oxoeremophilane(4)、(+)-9β,10β-epoxyeremophila-11(13)-en(5)、4(14)-eudesmene-8α,11-diol(6)、12,15-dioxo-selina-4,11-dien(7)、2β,8aα-dihydroxy-11-en-eremophilane(8)。其中化合物1和2为新化合物,通过计算ECD确定了其绝对构型。化合物1、4、6~8在20μmol·L浓度时能显著改善牛磺胆酸(TCA)诱导的GES-1人胃黏膜细胞损伤,其细胞保护率分别为23.51%±2.79%、16.10%±1.25%、24.45%±4.89%、17.48%±2.93%、21.44%±2.39%。Eight sesquiterpenoids were isolated from petroleum ether extract of Aquilariae Lignum Resinatum by various column chromatography techniques including silica gel,ODS,and semi-preparative HPLC.Their structures were identified on the basis of physicochemical properties,UV,IR,MS,and NMR spectroscopic data as(4S,5S,7R,10S)-5,7-dihydroxy-11-en-eudesmane(1),(7R,10S)-eudesma-4-en-11,15-diol(2),(2R,4S,5R,7R)-2-hydroxyeremophila-9,11-dien-8-one(3),7α-H-9(10)-ene-11,12-epoxy-8-oxoeremophilane(4),(+)-9β,10β-epoxyeremophila-11(13)-en(5),4(14)-eudesmene-8α,11-diol(6),12,15-dioxo-selina-4,11-dien(7),and 2β,8 aα-dihydroxy-11-en-eremophilane(8).Compounds 1 and 2 are new compounds,and their absolute configurations were determined by calculating ECD.Compounds 1,4,and 6-8 could significantly improve taurocholic acid(TCA)-induced gastric mucosal GES-1 cell injury at a concentration of 20μmol·L,and the cell protection rates were 23.51%±2.79%,16.10%±1.25%,24.45%±4.89%,17.48%±2.93%,and 21.44%±2.39%,respectively.

关 键 词：沉香 化学成分 倍半萜 胃黏膜细胞保护 

分 类 号：R284.1[医药卫生—中药学]