金环蛇(Bungarus fasciatus)蛇毒类心脏毒素的纯化及生化分析  

Study on Venom of Bungarus fasciatus,The Purification and Biochemical Analysis of Cardiotoxic Principles

作　　者：肖昌华[1] 张洪基[1] 雷克健 Xiao Changhua;Zhan Hongji;Lei Kejian(Kunming Institute of Zoology,Academia Sinica;Institute of Biophysics,Academia sinica)

机构地区：[1]中国科学院昆明动物研究所 [2]中国科学院生物物理研究所

出　　处：《Zoological Research》1981年第1期49-54,共6页动物学研究（英文）

摘　　要：金环蛇(Bungarus fasciatus)蛇毒经磷酸纤维素P11柱层析分离所得的第8峰(即类心脏毒素),再经磺乙基—葡聚糖凝胶G—25柱层析分离得8A、8B。再将8A、8B分别经葡聚糖凝胶G—50柱层析,得纯化的类心脏毒素A、B。经聚丙烯酰胺凝胶电泳鉴定为单一的条带。无酶活性。其氨基酸组成及含量基本与Shiau Lin 1975等所测定的结果一致。Fractionation of venom of Bungarus fasciatus was performed on cellulose phosphate p11 by column chromatography. Peak VIII (cardiotoxic principles) was further isolated on SE-Sephadex G-25 and divided into V111A and V111B. Purified cardiotoxic principle A and B were obtained by column chromatography of V111A and V111B on Sephadex G-50. A single band without enzyme activity was observed by polyacrylamide gel electrophoresis. The contents and composition of amino-acid are similar to those tested by Shiau Lin et al.

关 键 词：心脏毒素 葡聚糖凝胶 柱层析分离 生化分析 蛇毒类 金环蛇 聚丙烯酰胺凝胶电泳 的纯化 

分 类 号：R28[医药卫生—中药学]