蝮蛇Agkistrodon halys Pallas蛇毒纤溶酶对纤维蛋白质的作用  

THE ACTION OF THE FIBRINOLYTIC ENZYME OF THE VENOM OF AGKISTRODON HALYS (PALLAS) ON FIBRINOGEN

作　　者：阮长耿[1] 陈重坤 陈悦书[1] 于力 涂光俦 许金菊[2] 冉永禄[2] Ruan Chang-geng;Chen Chong-kun;Chen Yue-shu;Yu li;Tu Guang-chou;Xu Jin-ju;Ran Yong-lu(Department of Blood Diseases,Suzhou Medical College;Kunming Institute of Zoology,Academia Sinica)

机构地区：[1]苏州医学院血液病研究室 [2]中国科学院昆明动物研究所

出　　处：《Zoological Research》1981年第2期163-167,I0003,共6页动物学研究（英文）

摘　　要：应用SDS—聚丙烯酰胺凝胶电泳的方法证明蝮蛇毒纤溶酶对人体纤维蛋白原的α(A)链和β(B)链都有作用。借助酸性聚丙烯酰胺凝胶电泳观察到人纤维蛋白原经蝮蛇毒纤溶酶水解后,产生一系列分子量大小不等的碎片X′、Y′、D′和E′。By means of SDS (sodium dodecyl sulfate)-polyacrylamide gel electrophoresis it was demonstrated that the fibrinolytic enzyme of Agkistrodon halys(Pallas) venom not only degrades the a chain but also degrades the chain of human fibrinogcn. A series of the fragments with different molecular weights, X’, Y’, D’, E’, could be observed electrophoretically on acid polyacrylamidc gels after degradjng fibrinogen. The mechanism of the hydrolytic action of human fibrinogen by the fibrinolytic enzyme has been discussed.

关 键 词：人纤维蛋白原 蛇毒纤溶酶 纤维蛋白质 分子量大小 

分 类 号：R28[医药卫生—中药学]