灵长类淋巴细胞亚群的研究——Ⅱ.猕猴外周血淋巴细胞的非特异性酯酶活性  

STUDIES ON LYMPHOCYTE SUBPOPULATIONS IN PRIMATES II.NONSPECIFIC ESTERASE ACTIVITY OF PERIPHERAL BLOOD LYMPHOCYTES OF MACAQUES

作　　者：宋宝云 贲昆龙[1] 戴伟 邹如金[1] Song Baoyun;Ben Kunlong;Dai wei;Zhou Rujin(Kunming Institute of Zoology, Academia Sinica)

机构地区：[1]中国科学院昆明动物研究所

出　　处：《Zoological Research》1983年第3期261-266,I0002,共7页动物学研究（英文）

摘　　要：以酸性α——醋酸萘酯酶(ANAE)染色表明,恒河猴外周血涂片和淋巴细胞悬液片的ANAE阳性率分别为82.9±5.9%和84.6±4.2%熊猴、豚尾猴和红面猴也均在85%左右。用E_(AET)(AET处理过的绵羊红细胞)、ZYC(酵母多糖补体复合物)分别与ANAE一起进行双标记,或三者混合标记,发现T细胞、B细胞和裸细胞中分别有95.5±1.8%、52.8±13.0%和78.7±9.2%为ANAE阳性。提示ANAE染色并不能作为这些动物的特异性的T细胞鉴定方法。Acid a-naphthyl acetate eslerase (ANAE) positive lymphocytes in peripheral blood smears and lymphocyte suspension cytocentrifuge preparations of Macaca mulatta were 82.9+5.9% and 84.6 + 4.2%,respectively.Of M.assamensts,M.nemestrina and W.speciosa also were about 85%.By double marking with ANAE and EAET (AET-treated sheep red blood cells) or ZYC (zymosan-yeast-complement complex) and by triple marking with them simultaneously,ANAE positive cells in T,B and null cells of M.mulatia were 95.5+1.8%,52.8 + 3.0% and 78.7?.2%,respectively.The results indi that ANAE staining was not a specific method for marking of T cell in these monkeys.

关 键 词：淋巴细胞亚群 酵母多糖 红面猴 细胞悬液 非特异性酯酶 绵羊红细胞 外周血淋巴细胞 外周血涂片 

分 类 号：S85[农业科学—兽医学]