浙江产眼镜蛇毒胆碱酯酶的研究——Ⅲ、酶的疏水层析行为  

STUDIES ON THE CHOLINESTERASE FROM THE VENOM OF NAJA NAJA ATRA OF ZHEJIANG PROVINCE III.SOME HYDROPHOBIC CHROMATOGRAPHIC BEHAVIOUR

作　　者：余微明 邱雪贞[1] 屈贤铭[1] 陈峰 She Weiming;Qiu Xuezhen;Qu Xianming;Cheng Feng(Shanghai Institute of Biochemistry, Academia Sinica;Shanghai Institute of Occupational Disease and Industry Health)

机构地区：[1]中国科学院上海生物化学研究所 [2]上海市劳动卫生职业病研究所

出　　处：《Zoological Research》1983年第3期255-259,I0003,共6页动物学研究（英文）

摘　　要：本文报导了三种疏水层析吸附剂的制备,并比较了这几种吸附剂对浙江产眼镜蛇毒胆碱酯酶的吸附情况,它们都能吸附这种酶、烷基越长吸附得越牢。经此疏永层析后的酶,在聚丙烯酰胺凝胶电泳中依旧有6条同工酶带,提示这些同工酶的疏水性质相似。This paper reported the preparation of three hydrophobia chromatographic absorbents and the comparison of some hydrophobic chromatographic behaviour of the cholinesterase from the Venom of Naja Naja atra.The length of the "Spacer arm" affected the adsorption of the enzyme,the more extended the carbon chain of the "Spacer arm",the stronger the adsorption of the enzyme.The cholinesterase from hydrophobic chromatography gave six bands in disc gel electrophoresis too.That implied a similar of the hydrophobic region of the isoenzymes.

关 键 词：吸附情况 胆碱酯酶 眼镜蛇毒 疏水层析 聚丙烯酰胺凝胶电泳 同工酶 吸附剂 浙江 

分 类 号：X70[环境科学与工程—环境工程]