眼镜王蛇(Ophiophagus hannah)蛇毒四个神经毒组份的纯化和某些性质的研究  

PURIFICATION AND PROPERTIES OF FOUR NEUROTOXIC FRACTIONS FROM THE VENOM OF Ophiophagvs hannah

作　　者：孙欣 杨长久[1] 陈锡兰 雷克健 Sun Xin;Yang Changjiu;Chen Xilan;Lai Kejian(Kunming Institute of Zoology,Academia Sinica;Institute of Biophysics,Academia Sinica)

机构地区：[1]中国科学院昆明动物研究所 [2]中国科学院生物物理研究所

出　　处：《Zoological Research》1981年第4期363-370,共8页动物学研究（英文）

摘　　要：根据前文报导的方法(蔡景霞等人,1980),用羧甲基纤维素葡聚糖凝胶C25分离我国广西眼镜王蛇(Ophiophasus hannah)蛇毒,获得十七个蛋白组份。其中组份7、8、9和11蛋白回收率比较高,且具有明显的抗去极化神经肌肉阻遏作用。由于它们还含有磷酸单酯酶,磷酸二酯酶,5’—核苷酸酶和核糖核酸酶等酶活性,我们进一步用羧甲基纤维素CM32和葡聚糖凝胶G50纯化了组份7、8、9和11。通过聚丙烯酰胺凝胶电泳鉴定,它们均为单一条带,且具有神经肌肉阻遏作用,没有磷酸单酯酶等酶活性。随后测定了它们的氨基酸组成和N—末端氨基酸。根据离体大白鼠膈神经膈肌标本和去神经大白鼠膈肌标本实验和氨基酸组成测定结果表明,眼镜王蛇毒似乎既含长链神经毒也含短链神经毒,它们的作用部位是在神经肌肉突触后膜,对突触前膜没有影响,都是突触后神经毒素。From the venom of O.hannah,seventeen fractions had been isolated by column chromatography on CM-sephadex C25 as previously described.Among the seventeen fractions,.fractions 7,8,9 and 11 produced an antidepolarizing neuromuscular block.But they were contaminated with phosphomonoesterase,phosphodiesterase,5’-nucleotidase and ribonuclease.After re-chromatography on CM32 and gel filtration on sephadex G50,7-A,8-B,9 and 11 were confirmed to be homogenous by polyacrylamide gel electrophoresis.That si to say,they were free from phosphomonoesterase,etc.We determined the amino acid compositions and N-terminal groups of the four purified fractions.The experiment on rat phrenicdiaphragm and denerved rat diaphragm preparations in vitro and the determination of amino acid compositions suggested that O.hannah venom contained both long neurotoxins and thort ones.The site of action was found to be in the postsynapse instead of the in the presynapse.

关 键 词：突触前膜 作用部位 羧甲基纤维素 眼镜王蛇 神经毒 神经肌肉 核糖核酸酶 磷酸二酯酶 

分 类 号：R28[医药卫生—中药学]