眼镜王蛇(OPhiophagus hannah)毒中L-氨基酸氧化酶的分离纯化和理化性质的研究  

Purification and Properties of L-Amino Acid Oxidase from Ophiophagus hannah Venom

作　　者：雷克健 胡纫秋 王锦兰[1] Lei Ke-jian;Hu Ren-qiu;Wang Jin-Ian(Institute of Biophysics,Academia Sinica)

机构地区：[1]中国科学院生物物理研究所

出　　处：《Zoological Research》1981年第S01期43-50,共8页动物学研究（英文）

摘　　要：本文报道了眼镜王蛇毒中L-氨基酸氧化酶的纯化及某些性质。从眼镜王蛇毒中所分离L-氨基酸氧化酶在比活力及同王酶谱方面与从其他蛇毒来源的L-氨基酸氧化酶存在显著不同。 聚丙烯酰胺凝胶电泳结果显示该酶是均一的。根据梯度聚丙烯酰胺平板凝胶电泳测定其全酶分子量约为1.5×10^(5)道尔顿。在S、D、S梯度聚丙烯酰胺凝酸电泳上测得其分子量为7.3×10^(4)道尔顿。表明该酶是由相同分子量的二个亚基组成。紫外吸收光谱显示每个酶含2个FAD辅基。This paper reports the separation and some properties of L-amino acid oxidasc from Ophiophagus hannah Venom. Differences between L-amino acid oxidasc from Ophiophagus hannah and L-amino acid oxidasc from other sources in specific activity, and spectrum of isoenzymes arc noticed. The result of electrophoresis on polyacrylamide gel of the pure cnzyme showed that it is hemogeneous. The molecular weight determined by gradient polyacylamide gel slab (4-30%) is around 15x104 dalton. The subunit molecular weight determined by SDS gradient gel electrophoresis (4-30%) is around 7.3xl04 dalton. Therefore, the enzyme is composed of two subunits. U.V. spectrum showed that two FAD are contained in each molecular.

关 键 词：眼镜王蛇毒 紫外吸收光谱 道尔顿 聚丙烯酰胺 凝胶电泳 

分 类 号：R28[医药卫生—中药学]