眼镜蛇(Naja naja atra)神经毒素抗体和心脏毒素抗体的亲和层析纯化  

PURIFICATIONS OF ANTI-COBRA NEUROTOXIN ANTIBODY AND ANTI-COBRA CARDIOTOXIN ANTIBODY BY AFFINITY CHROMATOGRAPHY

作　　者：孙欣 龚潮梁 何其伟[1] 潘淑英 Sun Xin;Gong Chao-Hang;He Qi-wei;Pan Shu-yin(Kunming Institute of Zoololgy,Academia Sinica)

机构地区：[1]中国科学院昆明动物研究所

出　　处：《Zoological Research》1981年第S01期75-81,共7页动物学研究（英文）

摘　　要：用纯化的眼镜蛇神经毒素和心脏毒素分别免疫家兔,得到兔抗神经毒素血清和抗心脏毒素血清。神经毒素和心脏毒素通过其游离氨基固相化在Sepharose4B上,不改变它们的抗原性。用溴化氰活化的Sepharose 4B分别偶联神经毒素和心脏毒素作为亲和吸附剂,从兔抗神经毒素血清、兔抗心脏毒素血清和马抗眼镜蛇毒血清中分别提纯了神经毒素抗体和心脏毒素抗体。这些抗体对眼镜蛇毒进行免疫扩散时都只呈现单一的沉淀线,它们的亲和洗脱都十分类似,提纯的神经毒素抗体和心脏毒素抗体的中和效价比其兔抗毒血清分别提高17.5倍和35倍、比精制马抗眼镜蛇毒血清分别提高50倍和33.3倍。本文还讨论了如何提高抗眼镜蛇毒血清的效价和研制高效抗毒血清途径等问题,这些结果对蛇伤治疗可能是有好处的。Rabbils hyperimmunized with puaified neurotoxin and eardioloxin from Naja naja atra venom in Frcund’s complete adjuvant produced anti-neurotoxin antisera and anti-cardiotoxin antisera resjpectively.Cobra neurotoxin and cardiotoxin were immobilized on CNBr-acti valcd Sepharose-4B through their free amino groups without altering their their antigenic activities.The antineurotoxin antibody and anticardioioxin antibody were isolated from rabbit antisera and horse antiscra against cobra venom respectively by affinity chromatography.It showed a single precipitate band on immunodiffusion when both antibodies reacted with neurotoxin and cardiotoxin as well as with cobra venom respectively.Both antibodies were similar elute from Sepharosc 4B-neurotoxin and Sepharose 4B-cardiotoxin columns.Specific neutralizing capacities of the purified antineurotoxin antibody and anticardiotoxin antibody increased 17.5 and 35-fold over that of rabbit antiscra against cobra ncurotoxin and cobra cardiotoxin,and 50 and 33.3-fold over that of horse antisera against cobra venom respectively.It also discussed how to increase efficiency of antiscra and how to prepare highly efficient antisera.These results may lead to a substaintial improvement in the therapy of snakebites.

关 键 词：眼镜蛇神经毒素 心脏毒素 抗神经毒素 免疫扩散 游离氨基 眼镜蛇毒 溴化氰 固相化 

分 类 号：S85[农业科学—兽医学]