广西蛇毒中的ADP酶、ATP酶和5′核苷酸酶  

ADPASE, ATPASE AND 5’ NUCLEOTIDASE OF THE SNAKE VENOMS FROM GUANGXI

作　　者：魏琦[1] 周德义[1] 徐启瑜 Wei Qi;Zhou De-yi;Xu Qi-yu(Department of Biochemistry,Snake Venom Research Centre,Guangxi Medical College)

机构地区：[1]广西医学院蛇毒蛇伤研究室、生化组

出　　处：《Zoological Research》1981年第S01期143-146,共4页动物学研究（英文）

摘　　要：蛇毒含多种不同的磷酸酯水解酶类。其中5′核苷酸酶、非特异性磷酸单酯酶和磷酸二酯酶研究较多、较清楚。但ATP酶和ADP酶是否独立存在意见不一。Schenberg研究了白头蝰(Bitis ariatans)加蓬咝蝰(Bitis gabonica)美洲矛头腹(Bothrops jararaca)和南美响尾蛇恐怖亚种(Crotalus durissus terrificus)蛇毒的5′核苷酸酶、ADP酶及磷酸二酯酶,认为独立存在ATP酶。Brisbois等报告眼镜蛇(Naja naja atra)毒存在ATP酶。Pereira和Lima也指出ATP酶与磷酸二酯酶是两种不同的酶。然而,Pflevalerer和Ortanderl发现这两种酶的比值在分离纯化中不变,认为两种活力是同一种酶的多种功能。为了证实广西眼镜王蛇(Ophiophagus hannah)、眼镜蛇(Naja najaatra)和五步蛇(Agkistrodon acutus)蛇毒是否独立存在ADP酶和ATP酶.We have studied the distribution and contents of three classes of enzymes i. e. ADPase, ATPase and 5’ nuclcotidase in the snake venoms ot Ophiophagus Hannah, Naja naja atra and Agkistrodon acutus from Cuangxi, and researched the effects of denaturation of these enzymes at various temperatures, diffcrent pH values and with divalent cations. The results have shown that the confirmatory data on the existence in the three enzymes are independent from one another. Particularly, the aggregation of the platelets induced by ADP is deaggregated by one of these three snake venoms. No doubt that the ADPase can exist in these venoms. But it is unable to separate the correspondent enzymes from the snake venoms by polyacrylamidc gel clectrophoresis and ion exchange (CM-& DEAE-cellulose) chromatography.

关 键 词：磷酸二酯酶 水解酶类 ATP酶 眼镜王蛇 蛇毒 非特异性 磷酸单酯酶 ADP 

分 类 号：R28[医药卫生—中药学]