IL-15与NK细胞在不明原因复发性流产中的临床意义  

Clinical significance of IL-15 and NK cells in unexplained recurrent abortion

作　　者：谷秀霞 林宁[1] 戴凌 李晓红 赵琳[1] GU Xiu-xia;LIN Ning;DAI Ling;LI Xiao-hong;ZHAO Lin(Department of Obstetrics and Gynecology,Suzhou Science and Technology Town Hospital Affiliated to Nanjing Medical University,Suzhou 215000,Jiangsu,China)

机构地区：[1]南京医科大学附属苏州科技城医院妇产科,江苏苏州215000

出　　处：《川北医学院学报》2022年第9期1161-1165,共5页Journal of North Sichuan Medical College

基　　金：江苏省苏州市高新区医疗卫生科技计划项目(2019Z014)。

摘　　要：目的:探讨外周血白细胞介素(IL)-15与自然杀伤(NK)细胞亚群水平的表达和不明原因复发性流产(URSA)的关系,及其诊断URSA的价值。方法:以57例URSA患者为观察组,同期入院体检无不良孕产史的57例健康早孕者为对照组;酶联免疫法检测两组血清孕酮、绒毛膜促性腺激素β(HCG-β)、IL-15、肿瘤坏死因子-α(TNF-α)、血管内皮生长因子(VEGF)及趋化因子配体5(CCL5)水平,流式细胞仪检测两组NK细胞水平;分析IL-15与NK细胞亚群的相关性及诊断URSA效能。结果:观察组血清孕酮及HCG-β水平低于对照组(P<0.05),观察组IL-15水平高于对照组(P<0.05)。观察组NK细胞CD56^(+)CD16^(+)型高于对照组(P<0.05),NK细胞CD56^(+)CD16^(-)与CD56^(-)CD16^(+)型观察组低于对照组(P<0.05)。外周血IL-15水平和NK细胞CD56^(+)CD16^(+)型呈正相关性(r=0.560,P<0.05);外周血IL-15水平和NK细胞CD56^(+)CD16^(-)、CD56^(-)CD16^(+)型呈负相关性(r=-0.641、-0.571,P<0.05)。外周血IL-15与NK细胞的三种亚型水平对URSA的发生均有诊断学价值(AUC=0.680~0.927,P<0.05);IL-15与NK细胞的三种亚型联合诊断的AUC可达到0.942(P<0.05)。结论:外周血IL-15与NK细胞指标联合检测可增强URSA诊断的敏感度及特异性,临床监测外周血IL-15与NK细胞亚群可作为URSA诊断依据。Objective:To analyze the relationship between the expression of peripheral blood interleukin(IL)-15 and natural killer(NK)cell subsets and unexplained recurrent abortion(URSA),and the value of its combined diagnosis of URSA.Methods:The clinical data of 57 patients with URSA were collected as the observation group,57 healthy early pregnancies with no adverse pregnancy history were set as the control group.Serum progesterone and chorionic gonadotropinβ(HCG-β),IL-15,tumor necrosis factor-α(TNF-α),the levels of vascular endothelial growth factor(VEGF)and chemokine ligand 5(CCL5)were detected by enzyme-linked immunosorbent assay,the levels of NK cells in the two groups were detected by flow cytometry.The correlation between peripheral blood IL-15 and NK cell subsets and the diagnostic efficacy of URSA were analyzed.Results:Serum progesterone and HCG-βin the observation group were higher than those in the control group,the level of IL-15 in the observation group was lower than that in the control group(P<0.05).The CD56^(+)CD16^(+)types of NK cells in the observation group were higher than those in the control group,and the CD56^(+)CD16^(-)and CD56^(-)CD16^(+)types of NK cells in the observation group were significantly lower than those in the control group(P<0.05).The levels of IL-15 in peripheral blood was positively correlation with the CD56^(+)CD16^(+)type of NK cells(r=0.560,P<0.05),and the levels of IL-15 in peripheral blood was negatively correlation with the CD56^(+)CD16^(-)and CD56^(-)CD16^(+)types of NK cells(r=-0.641,P<0.05).The levels of il-15 and NK cell subtypes in peripheral blood were of diagnostic value for URSA(AUC=0.680~0.927,P<0.05).The AUC of the combined diagnosis of four indicators was 0.942(P<0.05).Conclusion:The combined detection of peripheral blood IL-15 and NK cell indexes can significantly enhance the sensitivity and specificity of diagnosis.Clinical monitoring of peripheral blood IL-15 and NK cell subsets can be used as the basis for the diagnosis of URSA.

关 键 词：白细胞介素15 自然杀伤细胞 不明原因复发性流产 

分 类 号：R714.21[医药卫生—妇产科学]