拉曼光谱技术在病原菌感染诊断中的应用进展  

Application progress on Raman spectroscopy in the diagnosis of pathogenic infections

作　　者：于淼[1,2] 王敬开 林恺铖 刘风翔 唐玉国 宋一之[2] YU Miao;WANG Jingkai;LIN Kaicheng;LIU Fengxiang;TANG Yuguo;SONG Yizhi(School of Biomedical Engineering(Suzhou),Division of Life Sciences and Medicine,University of Science and Technology of China,Suzhou 215163,Jiangsu Province,China;Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences,Suzhou 215163,Jiangsu Province,China)

机构地区：[1]中国科学技术大学生物医学工程学院(苏州)生命科学与医学部,江苏苏州215163 [2]中国科学院苏州生物医学工程技术研究所,江苏苏州215163

出　　处：《微生物与感染》2022年第2期102-109,共8页Journal of Microbes and Infections

基　　金：国家自然科学基金(32170173);姑苏领军人才计划(ZXL2021422);中国科学院科研仪器设备研制项目(YJKYYQ20200038)。

摘　　要：快速准确诊断感染性疾病病原体是遏制超级细菌传播和抗生素滥用的重要防线。目前,临床病原菌感染诊断十分依赖于培养手段,导致检测周期长达数日,不但影响了患者的及时诊治,还间接导致抗生素的滥用。拉曼光谱技术是一种无损、高灵敏的分子指纹图谱检测技术,近年来在生物学领域得到广泛应用,其具有的免培养、快速、高特异性、低成本等优点为病原菌感染的诊断提供了新方案。本文阐述了拉曼光谱技术的原理和特点,综述了其在病原菌鉴定和抗菌药物敏感性试验方面的应用进展。Accurate and rapid diagnosis of infectious pathogens is an important defense line to curb the spread of super bacteria and the abuse of antibiotics.At present,the clinical diagnosis of pathogenic infections relies heavily on culture methods,resulting in a long detection cycle of several days,which not only affects the timely diagnosis and treatment of patients,but also indirectly promotes the abuse of antibiotics.Raman spectroscopy is a non-destructive and highly sensitive molecular fingerprint detection technology,which has been widely used in biology in recent years.It provides a new scheme for the diagnosis of pathogenic infections because of its advantages of non-culture,rapid,high specificity and low cost.Based on the principles and characteristics of Raman spectroscopy technology,this paper expounds its application progress on the identification of pathogenic bacteria and antimicrobial susceptibility test.

关 键 词：拉曼光谱 细菌感染 病原菌鉴定 抗菌药物敏感性试验 

分 类 号：O657.37[理学—分析化学] R372[理学—化学]