五爪金龙抑制香蕉枯萎病有效成分的鉴定与抑菌效果分析  

Identification of the Effective Components of Ipomoea cairica in Inhibiting BananaFusarium Wilt and Analysis on Its Bacteriostatic Effect

作　　者：顾承真 高钰杰 邱子宿 吕晶 李婷 曾任森[2] GU Cheng-zhen;GAO Yu-jie;QIU Zi-su;LYU Jing;LI Ting;ZENG Ren-sen(College of Life Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Institute of Crop Resistance and Chemical Ecology,College of Crop Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学生命科学学院,福建福州350002 [2]福建农林大学作物科学学院作物抗性与化学生态学研究所,福建福州350002

出　　处：《福建农业科技》2022年第7期23-26,共4页Fujian Agricultural Science and Technology

基　　金：国家自然科学基金项目(31800332);福建省自然科学基金面上项目(2021J1095);福建农林大学杰出青年科研人才计划项目(xjq201922)。

摘　　要：探究五爪金龙抑制香蕉枯萎病病原菌的物质基础,为开发绿色、有效防治香蕉枯萎病的植物源农药提供先导化合物。以五爪金龙为研究对象,采用柱层析的方法对五爪金龙乙醇提取物的化学成分进行分离,用核磁共振波谱和质谱鉴定单体化合物的结构,并用抑菌圈法测定化合物在不同浓度下对香蕉枯萎病病原菌生长的影响。结果表明:从五爪金龙乙醇提取物中分离得到3个单体化合物,鉴定为东莨菪亭内酯、没食子酸、β-谷甾醇,其中东莨菪亭内酯在50μg·mL^(-1)的浓度下能很好地抑制香蕉枯萎病病原菌的生长。东莨菪亭内酯可作为先导化合物合成具有抑制香蕉枯萎病病原菌生长的新型农药。By exploring the material basis of Ipomoea cairica inhibiting the banana fusarium wilt pathogen(fusarium oxysporum f.sp.cubense),it could provide the lead compounds for the development of green and effective botanical pesticides against banana fusarium wilt disease.The chemical constituents of the ethanol extract of Ipomoea cairica were separated by using the method of column chromatography.The structures of the monomeric compounds were identified by nuclear magnetic resonance spectroscopy and mass spectrometry,and the effects of the compounds on the growth of banana fusarium wilt pathogen(fusarium oxysporum f.sp.cubense)at different concentrations were determined by using the bacteriostatic circle method.The results showed that three monomeric compounds were isolated from the ethanol extract of Ipomoea cairica,which were identified as scopoletin,gallic acid,andβ-sitosterol.And the scopoletin at the concentration of 50μg·mL^(-1) could well inhibit the growth of banana fusarium wilt pathogen(fusarium oxysporum f.sp.cubense).Therefore,scopolamine could be used as the lead compound to synthesize a new pesticide which could inhibit the growth of banana fusarium wilt pathogen(fusarium oxysporum f.sp.cubense).

关 键 词：五爪金龙 香蕉枯萎病 化学成分 抗菌活性 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]