自拟补肾祛瘀方对多囊卵巢综合征模型大鼠的作用及机制研究  

Efficacy and mechanism analysis of self-made Bushen Quyu decoction in treatment of polycystic ovary syndrome model rats

作　　者：牛永莉[1] 王艳[1] 孔丽 谢广妹[2] Yong-li Niu;Yan Wang;Li Kong;Guang-mei Xie(Department of Reproductive Medicine,Jiuquan City People's Hospital,Jiuquan,Gansu 735000,China;Reproductive Center,Gansu Provincial Maternity and Chind-Care Hospital,Lanzhou,Gansu 730050,China)

机构地区：[1]酒泉市人民医院生殖医学科,甘肃酒泉735000 [2]甘肃省妇幼保健院生殖中心,甘肃兰州730050

出　　处：《中国现代医学杂志》2022年第19期44-50,共7页China Journal of Modern Medicine

基　　金：甘肃省自然科学基金(No:17JR5RA028)。

摘　　要：目的探究自拟补肾祛瘀方治疗多囊卵巢综合征(PCOS)的疗效及机制。方法选取SD雌性大鼠,复制PCOS模型后随机分为对照组、模型组、西药组及联合组。对照组和模型组根据大鼠体重给予1 mL/(kg·d)氯化钠溶液灌胃,联合组每天根据体重给予补肾祛瘀汤剂11 g/kg和4.5 mg/kg二甲双胍灌胃,西药组仅给予4.5 mg/kg二甲双胍灌胃。药物干预后分别采用Western blotting、qRT-PCR、免疫组织化学法检测肿瘤坏死因子-α(TNF-α)、核因子κB(NF-κB)、核因子IκB(IκBα)的蛋白、mRNA及阳性表达。比较各组大鼠的体重及血清睾酮(T)、促卵泡激素(FSH)、黄体生成素(LH)水平。结果各组大鼠药物干预前的体重比较,差异无统计学意义(P>0.05);各组大鼠药物干预28 d后的体重比较,差异有统计学意义(P<0.05);进一步两两比较,模型组的体重高于另外3组(P<0.05),西药组高于联合组和对照组(P<0.05),联合组高于对照组(P<0.05)。各组大鼠血清T、FSH、LH水平比较,差异有统计学意义(P<0.05);进一步两两比较,模型组血清T、FSH、LH水平高于另外3组(P<0.05),西药组高于联合组与对照组(P<0.05),联合组高于对照组(P<0.05)。各组大鼠卵巢组织TNF-α、NF-κB、IκBα蛋白相对表达量比较,差异有统计学意义(P<0.05);进一步两两比较,模型组大鼠卵巢组织TNF-α、NF-κB蛋白相对表达量高于另外3组,IκBα蛋白相对表达量低于另外3组(P<0.05);西药组大鼠卵巢组织TNF-α、NF-κB蛋白相对表达量高于联合组与对照组(P<0.05),联合组高于对照组(P<0.05);西药组大鼠卵巢组织IκBα蛋白相对表达量低于联合组与对照组(P<0.05),联合组低于对照组(P<0.05)。各组大鼠卵巢组织TNF-α、NF-κB、IκBαmRNA相对表达量比较,差异有统计学意义(P<0.05);进一步两两比较,模型组大鼠卵巢组织TNF-α、NF-κB mRNA相对表达量高于另外3组,IκBαmRNA相对表达量低于另外3组(P<0.05);西药组大鼠卵巢组�Objective To explore the effect and mechanism of self-made Bushen Quyu Decoction in treatment of polycystic ovary syndrome(PCOS).Methods Six-week-old SPF SD female rats were selected and randomly divided into control group,model group,western medicine group,and combination group after letrozole modeling.After drug intervention,Western blotting,RT-PCR,and immunohistochemistry were used to detect the expression of tumor necrosis factor-α(TNF-α),nuclear factorκB(NF-κB),and nuclear factor IκB(IκBα).The body weight and serum testosterone(T),follicle-stimulating hormone(FSH),and luteinizing hormone(LH)were compared in each group before and after treatment.Results Before treatment,the differences in body weight of the rats in each group were tested by one-way ANOVA,and the difference was not statistically significant(PP>0.05).After treatment,the differences in the body weight of the rats in each group were statistically significant by one-way ANOVA test(P<0.05).Further pairwise comparisons showed that the body weight of the model group after treatment was higher than that of the other three groups(P<0.05),the body weight of the group was higher than that of the combined group and the control group(P<0.05),and the body weight of the combined group was higher than that of the control group(P<0.05).The differences between groups in abdominal aortic blood T,FSH and LH of rats in each group were statistically significant(P<0.05).Further pairwise comparison showed that T,FSH and LH in the model group were higher than those in the other three groups(P<0.05);at the same time,the western medicine group was higher than the combination group and the control group(P<0.05),and the combination group was higher than the control group(P<0.05).The differences in the protein expression,mRNA expression,and OD value of TNF-α,NF-κB,and IκBαin ovarian tissue of each group were statistically significant by one-way ANOVA test(P<0.05).Further pairwise comparison showed that the expression of TNF-α,NF-κB protein,mRNA expression,and

关 键 词：多囊卵巢综合征 模型大鼠 自拟补肾祛瘀方 疗效 机制 

分 类 号：R711.6[医药卫生—妇产科学]