二次展开薄层色谱法鉴别沉香挥发油  

Study on Identification of Agarwood Oil by Secondary Development Thin Layer Chromatography

作　　者：洪童奕 钟骏豪 罗雪冰 叶世楷 梁得诚 陈晓颖 章卫民 高晓霞 Hong Tongyi;Zhong Junhao;Luo Xuebing;Ye Shikai;Liang Decheng;Chen Xiaoying;Zhang Weimin;Gao Xiaoxia(School of Pharmacy,Guangdong Pharmaceutical University,Guangzhou 510006;Institute of Microbiology,Guangdong Academy of Sciences,State Key Laboratory of Applied Microbiology Southern China,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangzhou 510070,China)

机构地区：[1]广东药科大学药学院,广东广州510006 [2]广东省科学院微生物研究所华南应用微生物国家重点实验室广东省菌种保藏与应用重点实验室,广东广州510070

出　　处：《广东化工》2022年第17期197-201,189,共6页Guangdong Chemical Industry

基　　金：2020年广东省大学生创新创业训练计划项目(202010573018);广东省科学院国际科技合作引导专项(2019GDASYL-0503002);广东省教育厅普通高校重点科研项目(2020ZDZX1024)。

摘　　要：目的:建立沉香挥发油的薄层色谱鉴别方法,为其品质评价提供依据。方法:以苄基丙酮为对照品,对薄层色谱展开系统、展开过程、展距进行优化和方法学验证。结果:自制硅胶G薄层板,常温下二次展开,展开剂Ⅰ:正己烷-丙酮(95︰5)上行展开8cm,展开剂Ⅱ:石油醚(60~90℃)-乙酸乙酯(95︰5)上行展开18 cm,5%香草醛硫酸乙醇溶液为显色剂,105℃加热显色后日光下检视,薄层色谱斑点清晰、特征性好。结论:该方法操作简便、直观性强,专属性、稳定性和耐用性好,可用于水蒸气蒸馏制取的沉香挥发油鉴别。Objective:To establish the thin-layer chromatography(TLC)identification method for agarwood oil and provide the basis for the quality evaluation.Methods:Using benzylacetone as a reference substance,the development system,development process,and development distance of TLC were optimized.The methodological investigation of the determined TLC conditions was carried out.Results:With the method of secondary separation of the TLC,the sample solution was developed on the self-made 0.4 mm of silica G thin layer plate at room temperature,when n-hexane-acetone(95︰5)was used as the first developing solvent and the petroleum ether(60~90℃)-ethyl acetate(95︰5)was used as the second developing solvent,and then sprayed with 5%vanillin-sulfuric acid ethanol solution and heated to color in 105℃for the view under daylight.The obtained separation effect of TLC for agarwood oil was good,the spots were clear,and the characteristics were significant.Conclusion:The method is easy to operate,highly intuitive,and has good specificity,stability and durability,and can be used for the identification of agarwood volatile oil prepared by steam distillation.

关 键 词：沉香挥发油 二次展开薄层色谱 苄基丙酮 

分 类 号：TQ[化学工程]