超声提取法测定三七片中三七皂苷R_(1)、人参皂苷Rg_(1)和人参皂苷Rb_(1)的含量  

Determination of Notoginsenoside R_(1),Ginsenoside Rg_(1) and Rb_(1) in SanQi Tablets by Ultrasonic Extraction

作　　者：陈肇娜 吴丽荣 李丝红 CHEN Zhao-na;WU Li-rong;LI Si-hong(Quanzhou Medical College,Quanzhou 362000,China)

机构地区：[1]泉州医学高等专科学校,福建泉州362000

出　　处：《海峡药学》2022年第9期54-56,共3页Strait Pharmaceutical Journal

基　　金：泉州市科技计划项目(2018Z173)。

摘　　要：目的改进《中国药典》2020年版收载的三七片含量测定项下供试品的提取方法。方法以40%乙醇作为提取溶剂,超声提取三七片中的三种指标性皂苷成分三七皂苷R_(1)、人参皂苷Rg_(1)和人参皂苷Rb_(1),用高效液相色谱法测定含量。结果三七皂苷R_(1)在0.0081~0.3238 mg·mL^(-1)范围内线性关系良好(R^(2)=0.9999),平均回收率为98.99%,RSD=1.00%(n=6);人参皂苷Rg_(1)在0.0337~1.348 mg·mL^(-1)范围内线性关系良好(R^(2)=0.9991),平均回收率为99.07%,RSD=0.85%(n=6);人参皂苷Rb_(1).0327~1.307 mg·mL^(-1)范围内线性关系良好(R^(2)=0.9997),平均回收率为97.93%,RSD=1.51%(n=6)。结论本法简便、快速、安全、重现性好、结果稳定,可用于三七片含量测定中供试品的提取。OBJECTIVE To improve the extraction method of the SanQi tablets samlpe under the content determination process in Chinese Pharmacopoeia 2020.METHODS Notoginsenoside R_(1),Ginsenoside Rg_(1),and Ginsenoside Rb_(1) in SanQi tablets were extracted by ultrasonic with 40%ethanol as extraction solvent,and the concentrations were determined by HPLC.RESULTS The calibration curve of Notoginsenoside R_(1) showed good linearity over the entire concentration range of 0.0081-0.3238 mg·mL^(-1),with a correlation factor(R^(2))of 0.9999.The average recovery was 98.99%(RSD=1.00%,n=6).Ginsenoside Rg_(1) had a good linearity in the range of 0.0337^(-1).348 mg·mL^(-1)(R^(2)=0.9991),and the average recovery was 99.07%(RSD=0.85%,n=6).Ginsenoside Rb_(1) had a good linearity in the range of 0.0327^(-1).307 mg·mL^(-1)(R^(2)=0.9997),and the average recovery was 97.93%(RSD=1.51%,n=6).CONCLUSION Our method is simple,efficient,safe,reproducible and stable for the extraction of samples in the process of the content determination of SanQi tablets.

关 键 词：三七片 皂苷 超声提取法 含量测定 

分 类 号：R927[医药卫生—药学]