不同配比的黄芪-当归药对体外抗氧化性实验研究  

Effects of Different Ratios of Astragalus and Angelica on in vitro Experimental Study on Oxidation Resistance

作　　者：姜欢欢 周亚明 王礼丰 方欢乐[1] JIANG Huan-huan;ZHOU Ya-ming;WANG Li-feng;FANG Huan-le(Medical College of Xi'an Peihua University,Shaanxi Xi'an 710125,China)

机构地区：[1]西安培华学院医学院,陕西西安710125

出　　处：《广州化工》2022年第18期40-43,共4页GuangZhou Chemical Industry

基　　金：国家级大学生创新创业项目(No:202111400002);陕西省重点研发计划项目(2021SF-362)。

摘　　要：采用醇提法对黄芪、当归药对9种不同配伍比例进行提取,将提取液配置成含药材的6种浓度溶液,分别采用DPPH、ABTS和FRAP法检测9种不同配比黄芪-当归药对的抗氧化活性。研究发现当归∶黄芪=1∶0时对DPPH的清除效果最好,当归∶黄芪=3∶2时对ABTS+的清除能力最强,当归∶黄芪=1∶0对Fe3+的还原效果最好。实验结果表明当归比例高于黄芪比例的药对中抗氧化性越强,同时自由基的清除率随浓度的增加而增加。此研究为黄芪-当归配比用药提供了应用依据。Astragalus membranaceus and Angelica sinensis were extracted by alcohol extraction method in 9 different compatibility ratios.The extraction solution was prepared into 6 concentration solutions containing medicinal materials.The antioxidant activities of 9 Astragalus membranaceus and Angelica sinensis were detected by DPPH,ABTS and FRAP methods,respectively.It was found that Angelica∶Astragalus=1∶0 had the best scavenging effect on DPPH,angelica∶Astragalus=3∶2 had the strongest scavenging effect on ABTS,and Angelica∶Astragalus=1∶0 had the best reducing effect on Fe.The results showed that the ratio of Angelica sinensis was higher than that of Astragalus membranaceus,and the antioxidant activity was stronger,and the scavenging rate of free radicals increased with the increase of concentration.A basis was provided for the application of Astragalus and Angelica.

关 键 词：黄芪-当归 DPPH ABTS FRAP 抗氧化 

分 类 号：R284.2[医药卫生—中药学]