2020-2021年广西Victoria系乙型流感病毒的抗原性及基因特性分析  

Antigenic and genetic characteristics of Victoria lineage of influenza B virus in Guangxi during 2020-2021

作　　者：陈华凤[1,2] 康宁 张超[1,2] 王静 居昱 闭福银[1,2] Chen Huafeng;Kang Ning;Zhang Chao;Wang Jing;Ju Yu;Bi Fuyin(Guangxi Center for Disease Control and Prevention,Nanning 530028,Guangxi,China;Guangxi Key Laboratory of Major Infectious Disease Prevention and Control and Biosafety Emergency Response,Nanning 530028,Guangxi,China)

机构地区：[1]广西壮族自治区疾病预防控制中心,广西南宁530028 [2]广西重大传染病防控与生物安全应急响应重点实验室,广西南宁530028

出　　处：《疾病监测》2022年第8期1079-1087,共9页Disease Surveillance

基　　金：广西壮族自治区卫生和计划生育委员会自筹经费科研课题(No.Z2015457,No.Z2016433)。

摘　　要：目的 了解广西壮族自治区(广西)2020—2021年度B/Victoria(Bv)系流感病毒的抗原性及基因特征。方法 选取广西2020—2021年分离的36株Bv系流感毒株进行全基因组测序,并对血凝素(hemagglutinin,HA)和神经氨酸酶(neuraminidase,NA)基因进行序列拼接和基因特性分析。同时采用血凝抑制实验进行抗原性分析。结果 2020—2021年广西分离的Bv系流感病毒与WHO推荐的疫苗株B/Washington/02/2019相比,HA和NA基因的核苷酸同源性分别为99.1%~100.0%和99.1%~99.9%。系统进化树分析发现这36株Bv系流感毒株均属于1A(?3)B分支,与疫苗株属于同一分支,但广西毒株又进一步分为两个小分支。抗原性分析显示这两个小分支没有明显差异,均为疫苗株的类似株。氨基酸变异位点分析发现,HA蛋白携带R133G、N150K、N197D的氨基酸突变,变异涉及3个抗原决定簇。NA蛋白的酶活位点和神经氨酸酶抑制剂耐药位点均未发生突变。结论 2020—2021年度广西流行的Bv系流感病毒与WHO推荐的北半球疫苗株的组分匹配但存在持续变异,需要密切监测流行株的变异情况。Objective To understand the antigenicity and genetic characteristics of influenza B/Victoria(Bv) virus circulating in Guangxi between 2020 and 2021. Methods 36 strains of influenza Bv virus between 2020 and 2021 in Guangxi were selected for whole genome sequencing, the hemagglutinin(HA) and neuraminidase(NA) genes were assembled and analyzed. At the same time,antigenicity was analyzed by hemagglutination inhibition test. Results Compared with influenza vaccine strain B/Washington/02/2019, HA and NA genes of influenza Bv virus strains between 2020 and 2021 shared99.1%-100.0% and 99.1%–99.9% homology, respectively. Phylogenetic tree analysis found that 36 strains of influenza Bv virus all belong to the1A(?3)B branch, which the same branch with the vaccine strain. But the Guangxi virus strains were further divided into two small branches. Antigenicity analysis showed that the two small branches were not significantly different, and both were similar to the vaccine strains. Analysis of amino acid variation sites revealed that HA protein of the strains carried amino acid mutations of R133G, N150K, and N197D, and the variation involved three antigenic determinants.Neither the active site nor the neuraminidase inhibitor resistance site of NA protein had mutations. Conclusion The influenza Bv virus circulating in Guangxi between 2020 and 2021 matched the composition of the vaccine strain recommended by World Health Organization for the NorthernHemisphere. However, there is persistent variation, and it is still necessary to strengthen the surveillance of influenza virus variation to provide a scientific basis for prevention and control of influenza.

关 键 词：B/Victoria系流感病毒 神经氨酸酶 血凝素 基因特性 抗原性 

分 类 号：R211[医药卫生—中医学] R373