超高柱效十八烷基键合硅胶整体柱的制备及其性能研究  

Preparation and Properties of Ultra-high Column Effeciency Octadecyl Bonded Silica Monolithic Columns

作　　者：侯伟萍 平贵臣[1] 史全全 丁洪晶 巩霞 李金梅[1] 许辉[1] HOU W eiping;PING Guichen;SHI Quanquan;DING Hongjing;GONG Xia;LI Jinmei;XU Hui(College of Science,Inner Mongolia Agriculural University,Hohhot 010018,China)

机构地区：[1]内蒙古农业大学理学院,呼和浩特010018

出　　处：《内蒙古农业大学学报（自然科学版）》2022年第3期111-115,共5页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金项目(21665020);内蒙古自治区自然科学基金项目(2017MS0203)。

摘　　要：采用溶胶-凝胶法在毛细管内制备了具有表面键合十八烷基(C18)反相硅胶整体柱并进行了性能评价。同时对制备C18硅胶整体柱的反应条件进行了优化,柱效高达43万理论塔板数/m,这是目前为止硅胶整体柱最高柱效的报道。在该C18硅胶整体柱上2.7 min内分离了7种中性物质,表明所制得的C18硅胶整体柱具有柱效高和可进行快速分离的特点。Reversed-phase silica monolithic columns with octadecyl(C18)functionalities were prepared by sol-gel method and their performance was evaluated. Meanwhile,the reaction conditions for preparing C18 silica monolithic column were optimized. Up to430000 theoretical plates/m was obtained,which is the highest column efficiency so far. Seven neutral species were rapidly separated on these monolithic columns within 2. 7 min,demonstrating this C18 silica monolithic columns own ultrahigh column efficiency and high speed separation capability.

关 键 词：硅胶整体柱 溶胶-凝胶 高柱效 

分 类 号：O652.63[理学—分析化学]