饲料中添加雨生红球藻对尖吻鲈生长性能、抗氧化能力及免疫状态的影响  

Effect of Haematococcus pluvialis on growth performance,antioxidant capacity and immune responses of Lates calcarifer

作　　者：虞为[1,2,3] 陈雪晴 杨育凯 张燕娃[1,2] 黄小林 黄忠 李涛[1,2] 马振华 吴洽儿[1] 于刚 周传朋[1] 林黑着 YU Wei;CHEN Xueqing;YANG Yukai;ZHANG Yanwa;HUANG Xiaolin;HUANG Zhong;LI Tao;MA Zhenhua;WU Qia'er;YU Gang;ZHOU Chuanpeng;LIN Heizhao(South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation&Utilization,Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Fishery Ecology and Environment,Guangzhou 510300,China;Shenzhen Base of South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shenzhen 518121,China;Tropical Aquaculture Research and Development Center,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Sanya 572018,China;School of Life Science,Guangzhou University,Guangzhou 510006,China)

机构地区：[1]中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室/广东省渔业生态环境重点实验室,广东广州510300 [2]中国水产科学研究院南海水产研究所深圳试验基地,广东深圳518121 [3]中国水产科学研究院南海水产研究所热带水产研究开发中心,海南三亚572018 [4]广州大学生命科学学院,广东广州510006

出　　处：《南方水产科学》2022年第5期46-54,共9页South China Fisheries Science

基　　金：海南省自然科学基金项目(321QN0942);中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助(2018ZD01);广东省渔业生态环境重点实验室开放基金(FEEL-2020-5);中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金资助(2020TD54,2020TD55);亚洲合作资金项目——中国与南海周边国家现代渔业合作;广东省现代农业产业技术体系创新团队建设专项资金(2019KJ149,2019KJ143);国家重点研发计划项目(2019YFD0900402)。

摘　　要：雨生红球藻(Haematococcus pluvialis)富含天然虾青素等活性物质,为评价其在尖吻鲈(Lates calcarifer)饲料中的应用效果,采用雨生红球藻添加量分别为0(C)、0.2%(H1)、0.4%(H2)、0.6%(H3)、0.8%(H4)和1.0%(H5)的6组饲料,投喂尖吻鲈56 d。结果表明,H2、H3、H4和H5组尖吻鲈特定生长率和增重率均显著大于对照组(P<0.05);其中H3、H4和H5组全鱼粗脂肪含量均显著低于对照组(P<0.05);H2、H3、H4和H5组总抗氧化能力(T-AOC)显著高于对照组(P<0.05),H2、H3、H4和H5组的过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性显著低于对照组(P<0.05),处理组的丙二醛(MDA)浓度显著低于对照组(P<0.05);H3、H4和H5组实验鱼红细胞数(RBC)和血红蛋白(Hb)浓度显著高于对照组(P<0.05),H2、H3、H4和H5组的总胆固醇(TCHO)和甘油三酯(TG)浓度显著低于对照组(P<0.05),H3、H4和H5组的低密度脂蛋白胆固醇(LDL-C)浓度显著低于对照组(P<0.05);H2、H3、H4和H5组的溶菌酶(LZM)活性和免疫球蛋白M(IgM)浓度显著高于对照组(P<0.05),H3、H4和H5组的补体4(C4)浓度显著高于对照组(P<0.05)。回归分析结果表明,以增重率为目标,尖吻鲈饲料中雨生红球藻的最适添加量为0.66%。Haematococcus pluvialis is enriched in astaxanthin.To investigate its effects in the diet of Lates calcarifer,we fed L.calcarifer by six diets containing 0(C),0.2%(H1),0.4%(H2),0.6%(H3),0.8%(H4)and 1.0%(H5)H.pluvialis for 56 d.The results show that the specific growth rate(SGR)and weight gain rate(WGR)in H2,H3,H4 and H5 groups were significantly higher than those of the control group(P<0.05).Compared with the control group,the whole-body lipid protein content decreased significantly in the treatment groups(P<0.05).The dietary H.pluvialis improved the total antioxidant capacity(T-AOC)significantly(P<0.05),while the superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px)activities and malondialdehyde(MDA)content were significantly lower than those of the control group(P<0.05).Compared with the control group,the concentrations of red blood cell(RBC),hemoglobin(Hb)and complement 4(C4)increased(P<0.05)in H3,H4 and H5 groups,but the concentration of low-density lipoprotein cholesterol(LDL-C)decreased significantly(P<0.05).Compared with the control group,the concentrations of cholesterol(TCHO)and triglyceride(TG)decreased significantly(P<0.05),but the lysozyme(LYZ)activity and immunoglobulin(IgM)concentration increased significantly(P<0.05).The regression analysis on the WGR indicates that the recommended optimum dietary H.pluvialis level for the growth of L.calcarifer is 0.66%.

关 键 词：雨生红球藻 尖吻鲈 生长性能 免疫应答 

分 类 号：S965.332[农业科学—水产养殖]