维氏气单胞菌重要致病因子基因对环境条件的响应  

Responses of important virulence factors of Aeromonas veronii to environmental conditions

作　　者：王茜[1,2] 邓益琴 孙承文 林梓阳[2] 苏雯晓 刘梦瑶 程长洪 郭志勋[2] 冯娟[2] WANG Qian;DENG Yiqin;SUN Chengwen;LIN Ziyang;SU Wenxiao;LIU Mengyao;CHENG Changhong;GUO Zhixun;FENG Juan(Tianjin Agricultural University,TianJin 300392,China;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Development&Utilization,Ministry of Agriculture and Rural Affairs,Guangzhou 510300,China;Pearl River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Guangzhou 510380,China)

机构地区：[1]天津农学院,天津300392 [2]中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室,广东广州510300 [3]中国水产科学研究院珠江水产研究所,广东广州510380

出　　处：《南方水产科学》2022年第5期74-80,共7页South China Fisheries Science

基　　金：国家重点研发计划“蓝色粮仓科技创新”专项(2019YFD0900105-3);中国水产科学研究院中央级公益性科研院所基础科研业务专项资金资助(2020XT0407,2021SD15)。

摘　　要：鞭毛蛋白(Flagellin)、密度感应系统(Quorum sensing,QS)和Ⅲ型分泌系统(TypeⅢsecretion system,T3SS)是与维氏气单胞菌(Aeromonas veronii)致病性相关的重要因子,且受多种环境条件的共同调控。为研究外界环境因子变化对维氏气单胞菌致病因子的影响,以2株维氏气单胞菌的ascF、fliE和luxR致病因子为研究对象,通过实时荧光定量PCR技术从转录水平上探究了ascF、fliE和luxR基因对温度、pH、无机盐离子和培养转速等环境因子的响应。结果表明,维氏气单胞菌的3个基因对偏酸性环境(pH 6.5~7.0)、中低培养转速(150~210 r·min^(−1))和锌离子(Zn^(2+))、镁离子(Mg^(2+))为正响应;2株不同分子分型的维氏气单胞菌的3个基因的响应模式各有不同,说明了2株不同分子分型的维氏气单胞菌的致病机制可能有所差异;另外,维氏气单胞菌的重要致病因子受环境条件的调控,而呈现不同的变化规律,为进一步分析维氏气单胞菌的发病机制提供参考。As important pathogenic factors of Aeromonas veronii,flagellin,quorum sensing and Type Ⅲ secretion system(T3SS)are closely related to their pathogenicity and regulated by various environmental conditions.In order to study the effect of variation in external environmental factors on the pathogenic factors of A.veronii,we used the ascF,fliE and luxR factors of A.veronii as objects by real-time PCR method.The response of ascF,fliE and luxR genes to environmental factors such as temperature,pH,rotating speed and ions were explored at the transcription level.The results show that the three genes of A.veronii had a positive response to acidic environment(pH 6.5−7.0),low and medium speed(150−210 r·min^(−1)),Zn^(2+)and Mg^(2+).Theresponse patterns of the three genes of the two strains of A.veronii with different molecular types were different,indicating that the pathogenic mechanisms of A.veronii might be different.Besides,the important virulence factors of A.veronii were regulated by environmental conditions and showed different regular change.The results provide references for further research on the pathogenesis of A.veronii.

关 键 词：维氏气单胞菌 致病因子 实时荧光定量PCR 温度 PH 转速 

分 类 号：S941[农业科学—水产养殖]