柑橘主要砧木品种耐缺铁特性及FRO基因启动子特性分析  

Analysis of Iron Deficiency Tolerance and FRO Gene Promoter Characteristics of Main Citrus Rootstock Varieties

作　　者：张梅 王金娟 高美美 王莹 罗添鸣 冯邻 罗小英 李德谋 ZHANG Mei;WANG Jinjuan;GAO Meimei;WANG Ying;LUO Tianming;FENG Lin;LUO Xiaoying;LI Demou(Biotechnology Research Center,Southwest University,Chongqing 400715,China)

机构地区：[1]西南大学生物技术中心,重庆400715

出　　处：《西南大学学报（自然科学版）》2022年第10期37-47,共11页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金项目(30370984);重庆市科技局基础研究与前沿探索项目(cstc2016jcyjA0446).

摘　　要：分析了目前柑橘果园中常用砧木以及具砧木价值的17个柑橘砧木品种的耐缺铁特性,筛选出缺铁响应较强的铁螯合还原酶基因(FRO),分析了不同品种中受缺铁诱导铁螯合还原酶基因的上游调控区序列特征和缺铁诱导特性.结果表明:17个柑橘砧木品种中枳柚、资阳香橙和枳雀为耐缺铁类型,莽山野柑、红橘、朱橘、酸橘、扁平橘、土橘、枳、兴义大红袍和皱皮橘属于缺铁敏感型,枳橙、汕头酸橘、兰卜莱檬、枸头橙和黄木黎檬属于中间型.在克里曼丁橘(Citrus clementina)基因组中CcFRO1,CcFRO2和CcFRO3基因表达受缺铁诱导明显,在柑橘砧木品种中,CcFRO1和CcFRO3基因的上游调控序列存在较大差异.缺铁条件下,耐受型启动子在植株中表达特性发生明显改变,而敏感型启动子表达特性则不受缺铁影响.研究结果表明,柑橘砧木耐缺铁特性可能与铁螯合还原酶基因上游调控区有关,为选育耐缺铁柑橘砧木品种提供了理论基础.Seventeen common citrus rootstock varieties were used in this research.Analysis of iron deficiency tolerance and expression level of FRO homologous genes from Citrus clementina genome were performed.We cloned and analyzed the promoter sequence of CcFRO genes in response to iron deficiency,and analyzed relationship between genotype and iron deficient characteristics.The results indicated that the physiological characteristics of iron deficiency of 17 citrus rootstocks were divided into tolerant,intermediate and sensitive types,among which the tolerant type includes Poncirus trifoliata(L.)Raf×C.grandis Osb.Zhiyou,Citrus junos cv.Ziyangxiangcheng and C.ichangensis Swing×P.trifoliata.Zhique,the sensitive type includes C.mangsanensis Mangshanyegan,C.reticulata Blanco,Citrus erythrosa Hort.,C.sunki Hort.,C.depressa Hayata cv.Shiikuwasha,C.chuana Hort.,P.trifoliataa(L.)Raf Trifoliate orange,C.reticulate cv.Xingyidahongpao and C.vessucosa Hort.,and the intermediate type includes P.trifoliata(L.)Raf×C.sinensis Osb Carrizo citrange,C.reticulata Blanco cv.Shantoy suanju,C.limonia Osbeck cv.Rangpur lime,C.aurantium L.and C.limonia Osbeck cv.Huanglumeng.In Citrus clementina genome,there are five FRO homologous genes.Among them,the expression of CcFRO1,CcFRO2 and CcFRO3 genes were induced by iron deficiency.The upstream sequences of regulatory of CcFRO1 and CcFRO3 genes are quite different in citrus rootstock varieties,but only the upstream regulatory sequences of CcFRO3 was significantly correlated with the ability of iron deficiency tolerance.The expression increased in stem and root,and decreased in leaf and tip of shoot.However,the expression characteristics of the sensitive CcFRO3 promoter was not affected by iron deficiency.The results indicated that the iron deficiency tolerance of citrus rootstocks was related to the upstream region of regulatory of FRO gene.

关 键 词：柑橘砧木 耐缺铁特性 铁螯合还原酶基因 上游调控序列 

分 类 号：S666[农业科学—果树学]