甘蓝型油菜一次分枝角度主效基因染色体定位和候选基因挖掘  

Chromosome Location and Candidate Gene Mining of Major Genes for Primary Branching Angle in Brassica napus L.

作　　者：井付钰 王瑞 徐新福 曲存民 卢坤 李加纳 JING Fuyu;WANG Rui;XU Xinfu;QU Cunmin;LU Kun;LI Jiana(College of Agronomy and Biotechnology,Southwest University/Engineering Research Center of South Upland Agriculture,Ministry of Education,Chongqing 400715,China)

机构地区：[1]西南大学农学与生物科技学院/南方山地农业教育部工程研究中心,重庆400715

出　　处：《西南大学学报（自然科学版）》2022年第10期56-64,共9页Journal of Southwest University(Natural Science Edition)

基　　金：国家现代农业产业技术体系资助项目(CARS-12).

摘　　要：一次分枝角度是构建甘蓝型油菜理想株型的重要性状之一.本研究以分枝角度大的纯系黄花亲本62与分枝角度小的纯系粉花亲本77为亲本构建114个双单倍体(Double Haploid,DH)群体株系,通过基于测序的基因分型(Genotyping by Sequence,GBS)技术简化测序获得单核苷酸多态性(Single Nucleotide Polymorphism,SNP)数据,考察DH群体一次分枝角度表型,利用一般线性模型(General Linear Model,GLM)进行全基因组关联分析.结果表明:该群体GLM模型检测到8个显著SNP位点都分布在A07染色体,解释了25.21%~35.05%的表型变异.定位区间内分析和注释了49个基因,通过京都基因与基因组百科全书(KEGG)通路富集分析推测甘蓝型油菜一次分枝角度主效基因为编码GH3家族蛋白的BnaA07g01910D基因,此基因负责细胞繁殖扩增可能影响分枝角度表型.该研究结果有助于解析甘蓝型油菜分枝角度的遗传基础和调控机制,为指导分枝角度遗传改良奠定了基础.Primary branching angle is one of the important traits for constructing ideal plant type of Brassica napus L.In this study,a DH population of 114 lines was constructed by using pure line of yellow petal color parent 62 with large branching angle,and pure line of pink petal color parent 77 with small branching angle as parents.SNP data was obtained by GBS simplified sequencing.The primary branching angle phenotype of DH population was investigated.The genome-wide association analysis was performed using a general linear model(GLM).The results showed that eight significant SNP loci detected with GLM model in this population were distributed on chromosome A07,explaining 25.21%-35.05%of phenotypic variation.A total of 49 genes were analyzed and annotated in this region.KEGG analysis suggested that the major gene for primary branching angle in B.napus is BnaA07g01910D,which encodes GH3 family proteins.This gene is responsible for cell proliferation and amplification,possibly affect the branching angle phenotype.The study will help to analyze the genetic basis and regulation mechanism of branch angle in Brassica napus L.,and offer essential information for guiding genetic improvement of branch angle.

关 键 词：甘蓝型油菜 一次分枝角度 关联分析 染色体定位 

分 类 号：S634.3[农业科学—蔬菜学]