Genomics and proteomics of Apis mellifera filamentous virus isolated from honeybees in China  

作　　者：Dahe Yang Jun Wang Xi Wang Fei Deng Qingyun Diao Manli Wang Zhihong Hu Chunsheng Hou 

机构地区：[1]Institute of Apicultural Research,Chinese Academy of Agricultural Sciences,Beijing,100093,China [2]State Key Laboratory of Virology and National Virus Resource Center,Wuhan Institute of Virology,Center for Biosafety Mega-Science,Chinese Academy of Sciences,Wuhan,430071,China [3]Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha,410205,China

出　　处：《Virologica Sinica》2022年第4期483-490,共8页中国病毒学（英文版）

基　　金：the Open Research Fund Program of the State Key Laboratory of Virology of China(Grant No.2019IOV004);the key Research Program of Frontier Sciences of Chinese Academy of Sciences(Grant No.QYZDJ-SSW-SMC021);the National Natural Science Foundation of China(Grant No.31900154 and 31572471)。

摘　　要：Apis mellifera filamentous virus(Am FV)is a large DNA virus that is endemic in honeybee colonies.The genome sequence of the Am FV Swiss isolate(Am FV CH–C05)has been reported,but so far very few molecular studies have been conducted on this virus.In this study,we isolated and purified Am FV(Am FV CN)from Chinese honeybee(Apis mellifera)colonies and elucidated its genomics and proteomics.Electron microscopy showed ovoid purified virions with dimensions of 300–500×210–285 nm,wrapping a 3165×40 nm filamentous nucleocapsid in three figure-eight loops.Unlike Am FV CH–C05,which was reported to have a circular genome,our data suggest that Am FV CN has a linear genome of approximately 493 kb.A total of 197 ORFs were identified,among which36 putative genes including 18 baculoviral homologs were annotated.The overall nucleotide similarity between the CN and CH–C05 isolates was 96.9%.Several ORFs were newly annotated in Am FV CN,including homologs of per os infectivity factor 4(PIF4)and a putative integrase.Phylogenomic analysis placed Am FVs on a separate branch within the newly proposed virus class Naldaviricetes.Proteomic analysis revealed 47 Am FV virionassociated proteins,of which 14 had over 50%sequence coverage,suggesting that they are likely to be main structural proteins.In addition,all six of the annotated PIFs(PIF-0–5)were identified by proteomics,suggesting that they may function as entry factors in Am FV infection.This study provides fundamental information regarding the molecular biology of Am FV.

关 键 词：Apis mellifera filamentous Virus(AmFV) per os infectivity factor 4(PIF4) Genome sequence Proteomics Structural proteins Naldaviricetes 

分 类 号：S895[农业科学—特种经济动物饲养]