Rhodococcus ruber FIM-12菌株的分离鉴定及其生物活性研究  

Study on Isolation and Identification of Rhodococcus ruber FIM-12 Strain and Its Biological Activity

作　　者：陈洲琴 张祝兰[1] 程贤 严凌斌[1] 杨煌建[1] 连云阳[1] 张熠 Chen Zhouqin;Zhang Zhulan;Cheng Xian;Yan Lingbin;Yang Huangjian;Lian Yunyang;Zhang Yi(Fujian Provincial Institute of Microbiology,Fuzhou 350007,China;Fujina Instutute for Food and Drug Quality Control,Fuzhou 350025,China)

机构地区：[1]福建省微生物研究所,福建福州350007 [2]福建省食品药品质量检验研究所,福建福州350025

出　　处：《宁夏大学学报（自然科学版）》2022年第3期288-292,共5页Journal of Ningxia University(Natural Science Edition)

基　　金：福建省公益类基金资助项目(2020R10050014,2020R10050016)。

摘　　要：从海南省文昌市八门湾红树林土壤中分离一株产红色素菌株FIM-12,通过形态学、生理生化特征与分子生物学鉴定为赤红球菌(Rhodococcus ruber),命名为赤红球菌Rhodococcus ruber FIM-12.为考察赤红球菌提取物的生物活性,以小鼠为研究对象,通过灌胃的给药方式研究其毒性以及对免疫器官、巨噬细胞(MΦ)吞噬功能的影响.结果表明,灌胃的给药量ρ(LD_(50))>2.0 g/kg;一定质量浓度的赤红球菌提取物能增加免疫器官胸腺和脾脏的质量,提升白细胞数量,增强MΦ的吞噬活性.赤红球菌提取物的毒性低,能显著提高机体的免疫能力,具有一定的应用价值.A red pigment-producing strain FIM-12 was isolated from Bamenwan mangrove forest in Wenchang City,Hainan Province,and is identified as Rhodococcus ruber by morphological analysis,physiological and biochemical characteristics and molecular biology analysis,it is then named Rhodococcus ruber FIM-12.To study the biological activity of the Rhodococcus ruber extract,the toxicity and the effect on immune organs and macrophage(MΦ) phagocytosis were studied by gavage administration in mice.The results show that the administration of ρ(LD_(50)) is larger than 2.0 g/kg by gavage;a certain mass concentration of Rhodococcus ruber extract could increase the mass of immune organs thymus and spleen,increase the number of leukocytes,and enhance the phagocytic activity of MΦ.The Rhodococcus ruber extract prepared by FIM-12 is with low toxicity,can significantly improve the immune function of the body,and has good application and development value.

关 键 词：赤红球菌 分离鉴定 生物活性 

分 类 号：Q93[生物学—微生物学] R979.1[医药卫生—药品]