UPLC-MS法同时测定蓝藻中BMAA和DAB  

Simultaneous Determination of BMAA and DAB in Cyanobacteria by Ultra Performance Liquid Chromatography-Mass Spectrometry

作　　者：罗丛强 马泽民 陈龙 杨品红[1] LUO Cong-qiang;MA Ze-min;CHEN Long;YANG Pin-hong(Hunan Provincial Key Laboratory for Healthy Aquaculture and Product Processing around Dongting Lake,Hunan Engineering Research Center for Aquatic Biological Resources and Environmental Ecology,Hunan University of Arts and Science,Changde,Hunan 415000,China)

机构地区：[1]环洞庭湖水产健康养殖及加工湖南省重点实验室,水产生物资源与环境生态湖南省工程研究中心,湖南文理学院,湖南常德415000

出　　处：《环境监测管理与技术》2022年第5期49-52,共4页The Administration and Technique of Environmental Monitoring

基　　金：湖南省自然科学基金资助项目(2021JJ50135);常德市科技创新专项基金资助项目(2020G178,2020S006)。

摘　　要：用超高效液相色谱-质谱法(UPLC-MS)测定经6-氨基喹啉基-N-羟基琥珀酰亚胺基氨基甲酸酯(AQC)柱前衍生后的两种神经毒素β-N-甲氨基-L-丙氨酸(BMAA)和2,4-二氨基丁酸(DAB),通过优化试验条件,使方法在1.00μg/L~250μg/L范围内线性良好,BMAA和DAB的方法检出限分别为0.5μg/L和0.9μg/L。对空白蓝藻样品做低、中、高3个质量浓度水平的加标回收试验,BMAA和DAB的平均回收率分别为(88.2±4.0)%和(87.7±14.2)%,6次测定结果的RSD分别为2.6%~7.9%和2.1%~11.3%。Two neurotoxins,β-N-methylamino-L-alanine(BMAA)and 2,4-diaminobutyric acid(DAB)were determined by ultra-performance liquid chromatography-mass spectrometry(UPLC-MS),using pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate(AQC).By optimizing the test conditions,the method had good linearity in the range of 1.00μg/L~250μg/L.The detection limits of BMAA and DAB were 0.5μg/L and 0.9μg/L respectively.The average spiked recoveries of BMAA and DAB at low,medium and high concentration levels in blank samples were(88.2±4.0)% and(87.7±14.2)%,respectively,and the RSDs of 6 measurements were 2.6%~7.9% and 2.1%~11.3% respectively.

关 键 词：β-N-甲氨基-L-丙氨酸 2 4-二氨基丁酸 超高效液相色谱-质谱法 蓝藻 

分 类 号：O657.63[理学—分析化学]