Cross-reactivity of eight SARS-CoV-2 variants rationally predicts immunogenicity clustering in sarbecoviruses  

作　　者：Qianqian Li Li Zhang Ziteng Liang Nan Wang Shuo Liu Tao Li Yuanling Yu Qianqian Cui Xi Wu Jianhui Nie Jiajing Wu Zhimin Cui Qiong Lu Xiangxi Wang Weijin Huang Youchun Wang 

机构地区：[1]Division of HIV/AIDS and Sex-transmitted Virus Vaccines,Institute for Biological Product Control,National Institutes for Food and Drug Control(NIFDC),WHO Collaborating Center for Standardization and Evaluation of Biologicals,NHC Key Laboratory of Research on Quality and Standardization of Biotech Products and NMPA Key Laboratory for Quality Research and Evaluation of Biological Products,102629 Beijing,China [2]Jiangsu Recbio Technology Co.,Ltd.,215300 Taizhou,China [3]Graduate School of Chinese Academy of Medical Sciences&Peking Union Medical College,No.9 Dongdan Santiao,Dongcheng District,100730 Beijing,China [4]CAS Key Laboratory of Infection and Immunity,CAS Center for Excellence in Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,100101 Beijing,China

出　　处：《Signal Transduction and Targeted Therapy》2022年第8期3069-3079,共11页信号转导与靶向治疗（英文）

基　　金：supported by the National Key Research and Development Program of China(grant number 2021YFC0863300);General Program of the National Natural Science Foundation of China(grant number 82073621,32070678&82172244);Beijing Municipal Science and Technology Project(Z211100002521018);the Bill&Melinda Gates Foundation(Investment ID INV-006379).

摘　　要：A steep rise in Omicron reinfection cases suggests that this variant has increased immune evasion ability.To evaluate its antigenicity relationship with other variants,antisera from guinea pigs immunized with spike protein of SARS-CoV-2 variants of concern(VOCs)and variants of interest(VOIs)were cross-tested against pseudotyped variants.The neutralization activity against Omicron was markedly reduced when other VOCs or VOIs were used as immunogens,and Omicron(BA.1)-elicited sera did not efficiently neutralize the other variants.However,a Beta or Omicron booster,when administered as the 4th dose 3-months after the 3rd dose of any of the variants,could elicit broad neutralizing antibodies against all of the current variants including Omicron BA.1.Further analysis with 280 available antigen–antibody structures and quantification of immune escape from 715 reported neutralizing antibodies provide explanations for the observed differential immunogenicity.Three distinct clades predicted using an in silico algorithm for clustering of sarbecoviruses based on immune escape provide key information for rational design of vaccines.

关 键 词：RATIONAL ESCAPE neutral 

分 类 号：R563.1[医药卫生—呼吸系统]