N6-甲基腺苷的生物学功能及检测技术研究进展  

Research progress of N6-methyladenosine in biological function and detection technology

作　　者：孔玉洁 王晨[1] 何炳 KONG Yujie;WANG Chen;HE Bing(The First Affiliated Hospital of University of Science and Technology of China,Hefei 230001,Anhui,China)

机构地区：[1]中国科学技术大学附属第一医院,安徽合肥230001

出　　处：《检验医学》2022年第9期872-876,共5页Laboratory Medicine

摘　　要：N6-甲基腺苷(m6A)是哺乳动物细胞mRNA中丰度最高的一种动态可逆的修饰,由甲基化酶[甲基转移酶(METTL)3、甲基转移酶(METTL)14、肾母细胞瘤1关联蛋白(WTAP)]、去甲基化酶[肥胖基因相关蛋白(FTO)、α-酮戊二酸依赖性双加氧酶同源物(ALKBH)5]、甲基结合蛋白[YTH N6甲基腺苷RNA结合蛋白1(YTHDF1)、YTH结构域蛋白(YTHDC)1、YTHDC2]等协同调控。m6A甲基化修饰与人体的生长发育和多种疾病的发生发展密切相关。文章对m6A的分子机制、生物学功能和检测方法的最新进展进行综述,并对m6A检测的临床应用价值进行展望。N6-methyladenosine(m6A) is a dynamic reversible modification with the highest concentration of mRNA in mammalian cells. It is composed of methylases [methyltransferase like(METTL)3,METTL14,recombinant Wilms tumor 1 associated protein(WTAP)],demethylases [fat-mass and obesity-associated protein(FTO),alk B homolog(ALKBH)5],methyl-binding proteins [YTH N6-methyladenosine RNA binding protein 1(YTHDF1),YTH domain-containing(YTHDC)1,YTHDC2] and so on. The m6A methylation is related to the growth and development of human body and the occurrence and development of many diseases. In this review,the molecular mechanism,biological function,detection methods and the latest research progress of m6A are described,and the clinical application significance of m6A detection is prospected.

关 键 词：N6-甲基腺苷 RNA修饰 肿瘤 

分 类 号：R446.7[医药卫生—诊断学]