布鲁氏菌核酸检测方法研究进展  

Research Progress of Brucella Nucleic Acid Detection Methods

作　　者：包小雅 韩宇[2] 杨宇昊 翟景波[1,4,5] 孙国庆 BAO Xiao-ya;HAN Yu;YANG Yu-hao;ZHAI Jing-bo;SUN Guo-qing(Medical College,Inner Mongolia Minzu University,Tongliao 028043,China;Tongliao Center for Disease Control and Prevention,Tongliao 028000,China;College of Animal Science and Technology,Inner Mongolia Minzu University,Tongliao 028043,China;Key Laboratory of Zoonose Prevention and Control at Universities of Inner Mongolia Autonomous Region,Tongliao,028043,China;Brucellosis Prevention and Treatment Engineering Research Center of Inner Mongolia Autonomous Region,Tongliao,028043,China;Tongliao Animal Disease Prevention and Control Center,Tongliao 028000,China)

机构地区：[1]内蒙古民族大学医学院,内蒙古通辽028043 [2]通辽市疾病预防控制中心,内蒙古通辽028000 [3]内蒙古民族大学动物科技学院,内蒙古通辽028043 [4]人兽共患病防控自治区高等学校重点实验室,内蒙古通辽028043 [5]内蒙古自治区布鲁氏菌病防治工程技术研究中心,内蒙古通辽028043 [6]通辽市动物疫病预防控制中心,内蒙古通辽028000

出　　处：《内蒙古民族大学学报（自然科学版）》2022年第5期413-417,共5页Journal of Inner Mongolia Minzu University：Natural Sciences

基　　金：内蒙古自治区自然科学基金项目(2019MS08035);内蒙古自治区布鲁氏菌防治工程技术研究中心开放课题(MDK2018053)。

摘　　要：布鲁氏菌病是由布鲁氏菌感染引起的一种公认的世界性人兽共患病,随着分子生物学检测方法的不断发展完善,多种快速、特异、敏感的检测方法被应用于布鲁氏菌的诊断和分型。现介绍常用的布鲁氏菌的聚合酶链式反应(PCR)、实时荧光定量PCR(qPCR)、TaqMan荧光定量PCR、TaqMan MGB荧光定量PCR、单核苷酸多态性分型(SNP)、重组酶聚合酶链反应(RPA)、环介导等温扩增(LAMP)、多位点可变数目串联重复序列分析(MLVA)、基于CRISPR/Cas的快速检测方法等检测分型方法,旨为布鲁氏菌核酸检测提供依据。Brucellosis is a recognized worldwide zoonosis caused by Brucella infection.With the continuous devel-opment and improvement of molecular biological detection methods,a variety of rapid,specific and sensitive de-tection methods have been applied to the diagnosis and typing of Brucella.This paper reviews the common detec-tion and typing methods of Brucella such as polymerase chain reaction(PCR),real-time quantitative PCR(qP-CR),TaqMan quantitative PCR,TaqMan MGB fluorescent quantitative PCR,single nucleotide polymorphism(SNP)typing,recombinase polymerase amplification(RPA),loop-mediated isothermal amplification(LAMP),multi-locus variable number tandem repeat analysis(MLVA),CRISPR/Cas based rapid detection method and so on,so as to provide the basis for Brucella nucleic acid detection.

关 键 词：布鲁氏菌 TaqMan MGB荧光定量PCR SNP RPA LAMP CRISPR/Cas 

分 类 号：R378.5[医药卫生—病原生物学]