消瘤散的指纹图谱及含量测定  

Fingerprint and content determination of Xiaoliu Powder

作　　者：刘晓敏[1] 刘广洲[1] LIU Xiaomin;LIU Guangzhou(Affiliated Hospital of Jilin Medical College,Jilin 132013,China)

机构地区：[1]吉林医药学院附属医院,吉林吉林132013

出　　处：《沈阳药科大学学报》2022年第9期1080-1090,共11页Journal of Shenyang Pharmaceutical University

摘　　要：目的应用高效液相色谱法,建立了消瘤散的指纹图谱,并同时测定其中4种有效成分的含量。方法采用Venusil ASB C_(18)(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-体积分数0.1%甲酸水,梯度洗脱,流速为1.0 mL·min^(-1),柱温为35℃,检测波长为270 nm,进样量为5μL。结果建立了消瘤散的指纹图谱,以新橙皮苷为参照物峰,标定了15个共有峰。没食子酸、新橙皮苷、桂皮醛和丹皮酚质量浓度分别在5.1~51.0 mg·L^(-1)(r=0.9995)、20.0~200.0 mg·L^(-1)(r=0.9990)、10.5~105 mg·L^(-1)(r=0.9995)、15.4~153.5 mg·L^(-1)(r=0.9994)内呈现出良好的线性关系;平均回收率分别为101.2%、99.7%、99.4%和99.9%,RSD分别为1.1%、1.7%、1.6%和1.0%(n=9)。结论该方法为消瘤散的质量控制提供了理论依据。Objective To establish a fingerprint and to simultaneously determine the content of 4 active ingredients in Xiaoliu Powder using high performance liquid chromatography.Methods The chromatographic separation was performed on a Venusil ASB C_(18)(250 mm×4.6 mm,5μm)with gradient elution using acetonitrile and 0.1%formic acid water as mobile phase,and the flow rate was 1.0 mL·min^(-1).The column temperature was 35℃,the detection wavelength was 270 nm,and the injection volume was 5μL.Results The fingerprint of Xiaoliu San was established,and 15 common peaks were calibrated with neohesperidin as the reference peak.The linear relationship showed great within the range of 5.1-51.0 mg·L^(-1)(r=0.9995)for gallic acid,20.0-200.0 mg·L^(-1)(r=0.9990)for neohesperidin,10.5-105 mg·L^(-1)(r=0.9995)for cinnamaldehyde and 15.4-153.5 mg·L^(-1)(r=0.9994)for paeonol.The average recoveries were 101.2%(RSD=1.1%),99.7%(RSD=1.7%),99.4%(RSD=1.6%)and 99.9%(RSD=1.0%),respectively.Conclusion This method provides a theoretical basis for the quality control of Xiaoliu Powder.

关 键 词：消瘤散 高效液相色谱法 指纹图谱 含量测定 

分 类 号：R917[医药卫生—药物分析学]